中文摘要：本发明涉及一种快速检测转基因植物中CP4-EPSPS的方法，包括以下步骤：步骤一，胶体碳的制备；步骤二，适合PH值条件下胶体碳标记抗体的制备；步骤三，抗体包被至反应膜作为T线及二抗包被至反应膜的C线；步骤四，胶体碳试纸条的制备；步骤五，从待检植物中提取蛋白，制备蛋白提取液；步骤六，将检测试纸条插入蛋白提取液中反应；步骤七，适当反应时间后，进行肉眼判断结果。本发明采用标记了多克隆抗体E01的胶体碳垫与包被了多克隆抗体E01的硝酸纤维素膜和包被了羊抗兔IgG的硝酸纤维素膜制成检测试纸条；采用双抗体夹心免疫层析的原理，形成抗原-抗体复合物使结果肉眼可见。
外文摘要：NOVELTY - Method of detecting CP4- 5-enolpyruvylshikimate-3-phosphate (EPSPS) in transgenic plant, involves preparing labeling antibody using colloidal carbon, coating antibody on the reaction membrane C as T line and secondary antibody on the reaction film, obtaining colloidal carbon test strip, extracting target protein from the plant, preparing protein extracting liquid, reacting the mixture, and detecting the result with naked eyes.
USE - The method is useful for detecting CP4- 5-enolpyruvylshikimate-3-phosphate (EPSPS) in transgenic plant.
ADVANTAGE - The method enables rapid detection of CP4- 5-enolpyruvylshikimate-3-phosphate in transgenic plant.
DETAILED DESCRIPTION - INDEPENDENT CLAIMS are included for the following:
(1) test strip for detecting CP4- 5-enolpyruvylshikimate-3-phosphate (EPSPS) in transgenic plant; and
(2) method of preparing the test strip for detecting CP4- 5-enolpyruvylshikimate-3-phosphate (EPSPS) in transgenic plant.
主权项：快速检测转基因植物中CP4-EPSPS的方法，其特征在于，包括以下步骤：步骤一，胶体碳的制备；步骤二，适合PH值条件下胶体碳标记抗体的制备；步骤三，抗体包被至反应膜作为T线及二抗包被至反应膜的C线；步骤四，胶体碳试纸条的制备；步骤五，从待检植物中提取蛋白，制备蛋白提取液；步骤六，将检测试纸条插入蛋白提取液中反应；步骤七，适当反应时间后，进行肉眼判断结果。
申请号：CN201510954682.4
专利号：CN106885907-A
公开/公告号：CN106885907A
申请/专利权人：中国农业科学院油料作物研究所; 南京瑞启生物科技有限公司;
发明/设计人：吴刚; 李均; 武玉花; 李晓飞; 李允静; 朱莉; 王锐
分类号：G01N33/68
主分类号：G01N33/68
法律状态：实质审查
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