中文摘要：本发明公开了一种转基因大豆检测用多重PCR试剂盒及检测方法，可快速检测我国农业部批准进口的12种转基因大豆，包括MON87701、GTS40‑3‑2、MON89788、CV127、A2704、A5547、DP356043、DP305423、MON87769、MON87708、305423×40‑3‑2、MON87701×MON89788；本发明公开的多重PCR试剂盒基于“公共引物”介导的多重PCR方法，包括公共引物、多重引物、多重PCR体系检测；两步退火温度法提高多重PCR体系的特异性、敏感性，该多重PCR方法具有包容性，可继续增加检测靶标，有利于提高多重PCR的检测通量。本发明具有灵敏度高，经济、快速、操作简单等优点，可应用于食品检测。
外文摘要：NOVELTY - Multiplex PCR kit comprises common primer and chimeric primer of transgenic soybean detection. The common primer of comprises 17 nucleobases (SEQ ID NO: 1), given in the specification. The chimeric primer comprises 36-43 nucleobases (SEQ ID NOs: 2 to 18), given in the specification.
USE - Multiplex PCR kit for transgenic soybean detection and detection of transgenic soybean components in soy products (all claimed).
ADVANTAGE - The multiplex PCR kit increases detection target, and improves specificity and sensitivity of multiplex PCR system, and high sensitivity, economical, quick and simple process in food inspection.
DETAILED DESCRIPTION - An INDEPENDENT CLAIM is included for a method for detecting transgenic soybean by PCR amplification, which involves annealing in PCR reaction is carried out at 5-10 degrees C, and the final concentration of chimeric primer for the transgenic soybean is 8-80nM and 1600nM on common primer to obtain the desired product.
主权项：一种转基因大豆检测用多重PCR试剂盒，其特征在于：所述转基因大豆检测用多重PCR试剂盒包括公共引物、针对转基因大豆的嵌合引物；所述公共引物的序列为SEQ ID NO.1；所述针对转基因大豆的嵌合引物为SEQ ID NO.2至SEQ ID NO.18所示的核苷酸序列中的一种或几种：SEQ ID NO.1：CTCGTCAACTCCGCAAGSEQ ID NO.2：CTCGTCAACTCCGCAAGATATTGACCATCATACTCATTGCTGSEQ ID NO.3：CTCGTCAACTCCGCAAGTCACTTTCTTGAATTAGCTTGCTSEQ ID NO.4：CTCGTCAACTCCGCAAGCTTTTGCCCGAGGTCGTTAGSEQ ID NO.5：CTCGTCAACTCCGCAAGGCCCTTTGGTCTTCTGAGACTGSEQ ID NO.6：CTCGTCAACTCCGCAAGCGTCAGGAATAAAGGAAGTACAGTASEQ ID NO.7：CTCGTCAACTCCGCAAGATTTCTAACCTGGCTGCTATAGTTASEQ ID NO.8：CTCGTCAACTCCGCAAGTGTATAGGAAAGCGCAAACTGATGSEQ ID NO.9：CTCGTCAACTCCGCAAGATTAGGGTTTCAGCAGGTTCGTSEQ ID NO.10：CTCGTCAACTCCGCAAGAAAATACAAATTTAACACTTCATTGGSEQ ID NO.11：CTCGTCAACTCCGCAAGTACCAATGCTTAATCAGTGAGGCSEQ ID NO.12：CTCGTCAACTCCGCAAGGAATGCAACACACTGTAACAATTTGSEQ ID NO.13：CTCGTCAACTCCGCAAGAACCCTTCAATTTAACCGATGCSEQ ID NO.14：CTCGTCAACTCCGCAAGTTGCGAAGGATAGTGGGATTGTSEQ ID NO.15：CTCGTCAACTCCGCAAGGGCAGTAACTTGAAAGACTATGAACSEQ ID NO.16：CTCGTCAACTCCGCAAGATGAGAAGATGGTTTTTTCCAAGGTSEQ ID NO.17：CTCGTCAACTCCGCAAGGGTAATCTAAACATGCATGAGAAATGSEQ ID NO.18：CTCGTCAACTCCGCAAGTGTCGTTTCCCGCCTTCAG。
申请号：CN201710167334.1
专利号：CN106995841-A
公开/公告号：CN106995841A
申请/专利权人：苏州大学
发明/设计人：孙万平; 沈苏南; 王晓囡; 季建刚; 张丽君; 薛满
分类号：C12Q1/68; C12N15/11;
主分类号：C12Q1/68
法律状态：实质审查
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