中文摘要：本发明公开了一种区分转基因玉米和非转基因玉米的检测方法，采用太赫兹（THZ）检测的方式，对转基因与非转基因玉米压片样本进行了扫描，获取了转基因玉米和非转基因玉米的太赫兹（THZ）吸收谱。在扫描得到的太赫兹吸收谱中，通过观察玉米样本的扫描结果图，取颜色最深、特征最明显的8到10个样本点，并取它们的平均值，绘制各个品种玉米样本的太赫兹吸收谱。然后经预处理得到THZ频域谱，通过LS—SVM、BPNN、RF方法在matlab中对数据进行建模分析比较，从中找到一个最优的模型，以此来准确的区分转基因与非转基因玉米。本发明解决了目前检测转基因产品的高成本传统破损式化学检测方法制样繁琐、检测时间长、需要专业人员操作的问题，本发明THZ检测方法具有无损、效率高、快速等特点，可应用于区分转基因玉米和非转基因玉米的检测.
外文摘要：OVELTY - Distinguishing transgenic corn and non-transgenic corn by detection method comprises e.g. (i) crushing and fully grinding, (ii) drying, (iii) placing the dried transgenic maize corn and the dried non-transgenic corn flour on a tablet press, (iv) vacuuming, (v) measuring the measurement parameters, (vi) setting measurement conditions, (vii) set the frequency range, (viii) discharging the sample scanning result of the influence of the background, (ix) scanning the sheet sample, (x) pre-processing the obtained sample terahertz absorbing spectrum and finding one optimal solution.
USE - The method is useful for distinguishing transgenic corn and non-transgenic corn by detection method (claimed).
ADVANTAGE - The method: has complex sample preparation, long detection time and needs professional staff to operate, utilizes THZ detection method is nondestructive, has rapid speed and can be applied to distinguish transgenic corn and nontransgenic corn detection.
DETAILED DESCRIPTION - Distinguishing transgenic corn and non-transgenic corn by detection method comprises (i) placing non-transgenic maize particles in the two hemispheres of the high-throughput tissue ball mill, crushing and fully grinding to obtain non transgenic corn flour sample, (ii) taking non-transgenic corn samples and the powdered transgenic corn samples are separately placing in different vacuum dryers for complete drying to obtain dry sample of non-transgenic corn and dried transgenic corn samples, (iii) placing the dried transgenic corn and the dried non-transgenic corn flour on a tablet press, applying 15 KN force to the tableting machine to obtain sample of non-transgenic corn tablet and sample of transgenic corn tablet, (iv) placing transgenic corn tablet and non-transgenic corn tablet samples on a test bench of terahertz time domain spectroscopic detection system and vacuuming the test bed, (v) measuring the measurement ! paramete rs of the transgenic corn tablet and non-transgenic corn tablet samples in the terahertz measurement system, scanning area of the terahertz measuring system connects all the sample completely contained and ensuring the precision of measuring, (vi) setting measurement conditions, so that the thickness of the measured sample set is consistent with the actual sample thickness and ensuring the accuracy of the measured data, (vii) set the frequency range and analysis mode of the terahertz scan for the sample, (viii) terahertz scanning the background detection table, discharging the sample scanning result of the influence of the background, (ix) respectively for scanning the sheet sample to obtain sample terahertz absorbing spectrum, namely transgenic corn sample terahertz absorbing spectrum and non-transgenic corn sample terahertz absorbing spectrum, (x) pre-processing the obtained sample terahertz absorbing spectrum, finding one optimal solution, so as to accurately distinguishing the transgenic corn and non-transgenic corn.
主权项：一种区分转基因玉米与非转基因玉米的检测方法，其特征包括以下步骤：步骤一，研磨处理：将非转基因玉米颗粒放入高通量组织球磨仪的两个半球内，进行粉碎，充分研磨后得非转基因玉米粉样本；步骤二，干燥处理：将所述的非转基因玉米粉样本和购得的粉末状转基因玉米样本分别放入不同的真空干燥箱中进行充分干燥，得到干燥的非转基因玉米粉样本和干燥的转基因玉米粉样本；步骤三，压片处理：将所述干燥的转基因玉米粉和干燥的非转基因玉米粉置于压片机模具上，对压片机施加15KN的力，得到压片样本即非转基因玉米压片样本和转基因玉米压片样本；步骤四，将所述转基因玉米压片样本和非转基因玉米压片样本放置于太赫兹时域光谱探测系统的检测台上，并对实验台抽真空；步骤五，在所述太赫兹测量系统中输入转基因玉米压片样本和非转基因玉米压片样本的测量参数，使得太赫兹测量系统的扫描面积将所有待测样本完全包含，同时保证测量的精度；步骤六，设置测量条件，使得设置的测量样本厚度与实际样本厚度相一致，从而保证实验所测数据的准确性；步骤七，设置太赫兹对样本扫描的频率范围和分析方式；步骤八，对太赫兹检测台进行背景扫描，用以排除背景对样本扫描结果的影响；步骤九，然后再分别对所述压片样本进行扫描，获得样本太赫兹吸收谱，即转基因玉米压片样本太赫兹吸收谱和非转基因玉米压片样本太赫兹吸收谱；步骤十，对所得样本太赫兹吸收谱进行预处理，从中找到一个最优的方案，以此来准确地区分出转基因玉米与非转基因玉米。
申请号：CN201710188363.6
专利号：CN106959284-A
公开/公告号：CN106959284A
申请/专利权人：江苏大学
发明/设计人：李青林; 黄云林; 张晓东; 毛罕平; 左志宇; 倪纪恒
分类号：G01N21/3586
主分类号：G01N21/3586
法律状态：实质审查
点击下载：一种区分转基因玉米和非转基因玉米的检测方法