中文摘要：本发明涉及用于转基因大米内标蔗糖磷酸合酶基因（sucrose phosphate synthase，SPS）成分的精准定量检测的寡核苷酸引物探针，包含所述引物探针的试剂盒。本发明还涉及用于定量检测转基因大米内标基因SPS成分的数字PCR检测方法，所述方法包括使用针对转基因大米内标基因SPS的特异性寡核苷酸引物和荧光标记探针。本发明还涉及针对转基因大米品系特异性基因的特异性寡核苷酸引物和荧光探针在定量检测转基因大米内标基因SPS成分中的应用。使用本发明的数字PCR检测方法，能够精准灵敏地测定样品中转基因大米内标基因SPS成分含量，其检测绝对灵敏度可以达到1copy/μL。
外文摘要：The present invention relates to transgenic rice internal standard sucrose phosphate synthase gene (sucrose phosphate synthase, SPS) ingredient precise quantitative detection of oligonucleotide primer probe, comprising the primer probe of the kit. The invention also relates to a method for quantitative detection of transgenic rice internal standard gene SPS component digital PCR detection method, the method including the use of aiming at the SPS transgenic rice internal standard gene specific oligonucleotide primer and fluorescence-labeled probe. The invention also relates to against the transgenic rice strain specific gene specific oligonucleotide primers and fluorescent probe in the quantitative detection of transgenic rice internal standard gene SPS component in the application. The use of the present invention digital PCR detection method, can be accurately and sensitively determining the sample transfer gene of rice internal standard gene SPS ingredient content, its measure the absolute sensitivity can reach 1 copy / μl.
主权项：用于数字PCR方法检测转基因大米内标基因SPS成分的特异性寡核苷酸引物对和荧光标记探针组合物，其中所述引物对为上游引物SPS-F1 GAGAACAAGAAGCCCCTT CTGTCTCG（SEQ ID No.1），所述下游引物为SPS-R1 AAGGTACTAAA GCTTGAAAATCCTAAGGC（SEQ ID No.2）；所述探针为SPS-P1 CACATTCGGCAGTGAAACTCTTGAGCGCC（SEQ ID No.3）。
申请号：CN201610257608.1
专利号：CN107312818A
公开/公告号：CN107312818A
申请/专利权人：中国检验检疫科学研究院;
发明/设计人：陈颖; 邓婷婷; 黄文胜; 葛毅强; 吴亚君
分类号：C12Q1/68; C12N15/11;
主分类号：C12Q1/68
法律状态：实质审查
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