中文摘要：本发明提供一种评价转基因抗虫水稻水生环境安全性的方法，包括如下步骤：转基因稻田和非转基因稻田布置和管理；稻田水层和淤泥中外源蛋白的测定，包括样本预处理和外源蛋白的抽提和测定；稻田水层中浮游生物的分离，包括水样采集、沉淀分离浮游生物；稻田水层中浮游生物种类和丰度的测定，包括活体鉴定和数量计数；转基因抗虫水稻的种植对浮游生物的安全性评价，利用分析软件对浮游生物种类、数量、发生时期进行统计分析，得出多样性指数、均匀性指数等参数，并和常规稻田进行比较，从而对转基因抗虫水稻水生环境安全性进行评价。本发明对于系统评估转基因抗虫水稻的水生环境安全性具有实际的应用价值。
外文摘要：The invention provides a method for evaluating the safety of aquatic environment for genetically modified insect resistant rice. The method comprises the following steps of : arranging and managing genetically modified paddies and non-genetically modified paddies; determining foreign protein in water layers and silt of the paddies, which comprises pretreating samples and extracting and determining the foreign protein; separating plankton in the water layers of the paddies, which comprises collecting water samples and precipitating and separating the plankton; determining the kind and the abundance of the plankton in the water layers of the paddies, which comprises identifying living bodies and counting the number; and planting the genetically modified insect resistant rice and evaluating the safety of the plankton, performing statistical analysis on the kind, the number and the occurrence period of the plankton by using analysis software so as to obtain parameters of a diversity index, a uniformity index and the like, and comparing the parameters with those of conventional paddies so as to evaluate the safety of the aquatic environment for the genetically modified 53 insect resistant rice. The method disclosed by the invention has practical application value for systematically assessing the safety of the aquatic environment for the genetically modified insect resistant rice.
主权项：一种评价转基因抗虫水稻水生环境安全性的方法，其特征在于，包括如下过程：（1）转基因稻田和非转基因稻田布置和管理；（2）稻田水层和淤泥中外源蛋白的测定；（3）稻田水层中浮游生物的分离；（4）稻田水层中浮游生物种类和丰度的测定；（5）转基因抗虫水稻的种植对浮游生物的安全性评价；以上所述转基因稻田和非转基因稻田布置和管理包括如下步骤：1）选择地势平整、水源方便的田块3至5块，田块面积400-600m2，长宽比范围2∶1至1∶1，田周边及水源无生活污水污染；2）将步骤1）中的田块划分成小区，田块周围筑不透水田埂及硬化沟渠；为防止暴雨漫灌，田块周边建立良好排水沟；3）在步骤2）中的上游小区种植转基因抗虫水稻，下游小区种植非转基因水稻，中间两块非转基因小区用上游转基因小区的田水灌溉，两侧的两块小区直接用水源中的水灌溉；4）从移栽至成熟期，不施用化学农药，人工除草，中期不晒田。
申请号：CN201510470544.9
专利号：CN104982241B
公开/公告号：CN104982241B
申请/专利权人：华中农业大学
发明/设计人：王永模; 刘标
分类号：A01G7/00
主分类号：A01G7/00
法律状态：实质审查
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