一种转基因大豆WH8013转化事件外源插入载体的右侧翼序列

A transgenic soybean WH8013 transforms incident exogenous insertion vector of the right wing sequence

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中文摘要:本发明涉及一种转基因大豆WH8013转化事件外源插入载体的右侧翼序列、所述右侧翼序列的应用、引物对、PCR试剂盒以及引物对和PCR试剂盒的使用方法。其中,本发明所述右侧翼序列的核苷酸序列如SEQ ID NO:4所示,其能够特异性地指示第8号染色体第21177109位核苷酸处插入pCHAL1的T‑DNA片段的转基因大豆。本发明首次测序公布了转基因大豆WH8013转化事件外源插入载体的右侧翼序列,建立了转基因大豆WH8013特异性定性PCR检测方法,该方法灵敏,准确,简单,可靠,具有广阔的市场前景。
外文摘要:The invention relates to a transgenic soybean WH8013 transforms incident exogenous insertion vector of the right wing sequence, application of the right flanking sequence, primers, PCR kit and PCR primer pair and method of use of the kit. Wherein the invention the right flanking sequence as the nucleotide sequence of SEQ ID NO : 4 as shown, it can be specifically indicating section 8 chromosome article 21177109 inserted at the nucleotide position of the pCHAL 1 T - DNA fragment of transgenic soybean. The invention first sequencing discloses transgenic soybean WH8013 transforms incident exogenous insertion vector of the right wing sequence, the establishment of transgenic soybean WH8013 specific qualitative PCR detection method, the method is sensitive, accurate, simple, reliable, and has wide market prospect.
主权项:一种转基因大豆WH8013转化事件外源插入载体的右侧翼序列,其特征在于,其核苷酸序列如SEQ ID NO:4所示。
申请号:CN201710830540.6
专利号:CN107460193A
公开/公告号:CN107460193A
申请/专利权人:吉林省农业科学院
发明/设计人:于志晶; 尚丽霞; 蔡勤安; 马瑞; 庄丹; 任鹏飞; 王丹
分类号:C12N15/11; C12Q1/68;
主分类号:C12N15/11
法律状态:实质审查
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  1. 编译服务:农产品质量安全
  2. 编译者:郭婷
  3. 编译时间:2018-01-02