中文摘要：本发明公开一种鉴别植物转基因和非转基因的装置及方法，包括发泡载板，所述发泡载板上表面和下表面均设置有指槽，所述发泡载板上表面设置有嵌入槽，所述嵌入槽内设置有正电荷薄膜，所述嵌入槽的槽壁上镶嵌有连接链母链，所述正电荷薄膜的边沿上均设置有翻边形成槽状，所述翻边上均设置有与连接链母链相配对的连接链子链，所述正电荷薄膜上设置有储物槽，所述储物槽呈矩形阵列分布，所述储物槽内设置有外源基因探针，所述发泡载板上设置有用于盖住嵌入槽的护罩，所述护罩为透明设置，所述护罩上设置有插入部；该鉴别植物转基因和非转基因的装置可以对多种转基因的外源性基因作出快速、准确和定性的判断分析，可以有效的满足市场的需求。
外文摘要：   NOVELTY - Device comprises foamed carrier plate. The upper surface and the lower surface of the foamed carrier plate are provided with a finger groove. The upper surface of foamed carrier plate is equipped with an embedding groove. The embedding groove is provided with a positive charged film. The groove wall of the embedding groove is embedded with a connecting chain main chain. Edges of the positive charged film are provided with a flanges forms groove. The flanges are provided with a connecting chain matched with the connecting chain main chain. The positive charged film is set with a storage tank.    USE - The device is useful for identifying transgenic and non-transgenic plants (claimed).    ADVANTAGE - The device has fast, accurate and qualitative judging and analyzing capacity.    DETAILED DESCRIPTION - Device comprises foamed carrier plate. The upper surface and the lower surface of the foamed carrier plate are provided with a finger groove. The upper surface of foamed carrier plate is equipped with an embedding groove. The embedding groove is provided with a positive charged film. The groove wall of the embedding groove is embedded with a connecting chain main chain. Edges of the positive charged film are provided with a flanges forms groove. The flanges are provided with a connecting chain matched with the connecting chain main chain. The positive charged film is set with a storage tank. The storage tank is distributed in rectangular array. The storage tank is provided with a exogenous gene probe. The foamed carrier plate is provided with a protection cover for covering the embedding groove. The protection cover is transparent. The protective cover is provided with an inserting part. The foamed carrier plate is provided with an inserting hole matching with the inserting part. The inserting part is provided with insertion hole, which is through hole. The insertion part is inserted in the insertion hole. The foam carrier and a shield are detachably connected by the inserting part and inserting hole. The foamed carrier comprises 15-17 pts. wt. green silicon carbide, 44-48 pts. wt. carbon fiber, 57-75 pts. wt. glass fiber, 5-11 pts. wt. hollow glass bead, 15-20 pts. wt. boron fiber, 18- 28 pts. wt. terpene resin, 90-133 pts. wt. rosin, 44-53 pts. wt. copa resin, 1-4 pts. wt. magnesium stearate, 1-3 pts. wt. tartaric acid, 13-15 pts. wt. gas phase white carbon black, 19-23 pts. wt. Arabic gum and 6-9 pts. wt. sodium bicarbonate. The hole wall of the insertion hole is provided with a rubber pad. The rubber pad is adhered with hole wall of the inserting hole. The quantity of rubber pad is more than one. The rubber pads are distributed in an annular array. An INDEPENDENT CLAIM is also included for identifying transgenic and non-transgenic plants comprising (i) providing 35S promoter, FMV35S promoter, Nos terminator, neomycin phosphotransferase II (NPTII) gene, CryI (AC) gene, Cry9c gene, modified CP4-5-enolpyruvulshikimate-3-phosphate synthase (EPSPS) gene, 35s-CTP2, CP4-EPSPS gene, glucose oxidase (GOX) gene, bar gene, barnase gene, barstar gene, CryIA (a) gene, lectin gene, napin gene, Sad1 gene, Zein gene placing into storage groove to obtain exogenous gene probe, and (ii) providing 5 mu g polymerase chain reaction amplification product, denaturing in pure water at 100 degrees C by boiling for 5 minutes, ice cooling for 3-5 minutes to obtain the pre hybridization solution for exogenous gene probe hybridization, wetting exogenous gene probe using 0.25 M phosphate buffer solution, placing exogenous gene probe into pre-hybridization liquid, performing pre hybridization for 10-12 hours at 60 degrees C, washing using washing film liquid for 2 times, each time for 20 minutes, dyeing for 5-10 minutes, rinsing using tris EDTA (TE) buffer solution for 5-10 minutes, repeating for 2 times, and detecting under 254 nm ultraviolet light or under fluorescence microscope to obtain the result.    DESCRIPTION OF DRAWING(S) - The drawing shows a schematic representation of the partial sectional view of the device. 
主权项：1.一种鉴别植物转基因和非转基因的装置，其特征在于：包括发泡载板，所述发泡载板上表面和下表面均设置有指槽，所述发泡载板上表面设置有嵌入槽，所述嵌入槽内设置有正电荷薄膜，所述嵌入槽的槽壁上镶嵌有连接链母链，所述正电荷薄膜的边沿上均设置有翻边形成槽状，所述翻边上均设置有与连接链母链相配对的连接链子链，所述正电荷薄膜上设置有储物槽，所述储物槽呈矩形阵列分布，所述储物槽内设置有外源基因探针，所述发泡载板上设置有用于盖住嵌入槽的护罩，所述护罩为透明设置，所述护罩上设置有插入部，所述发泡载板上设置有与插入部相契合的插入孔，所述插入孔为通孔，所述插入部插入插入孔内，所述发泡载板和护罩通过插入部和插入孔可拆卸连接，所述发泡载板由按重量份数配比的绿碳化硅15-17份、碳纤维丝44-48份、玻璃纤维丝57-75份、空心玻璃微珠5-11份、硼纤维15-20份、萜烯树脂18-28份、松香90-133份、柯巴树脂44-53份、硬脂酸镁1-4份、酒石酸1-3份、气相白炭黑13-15份、阿拉伯胶19-23份和碳酸氢钠6-9份组成,所述插入孔的孔壁上设置有橡胶垫，所述橡胶垫与插入孔的孔壁粘合，所述橡胶垫设置有一个以上，所述橡胶垫呈环形阵列分布。
申请号：CN201711097010.1
专利号：CN107641593A
公开/公告号：CN107641593A
申请/专利权人：南通科技职业学院;
发明/设计人：吕峰; 陈敏; 石小军; 蔡银杰; 郑兴国
分类号：C12M1/00; C12Q1/6816;
主分类号：C12M1/00
法律状态：实质审查
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