中文摘要：本发明提供了一种转基因水稻G6H1品系特异性定量PCR检测方法，包括以下步骤：A1、设计合成引物和荧光探针；所述引物的序列如SEQ ID No.1和SEQ ID No.2所示，所述荧光探针的序列如SEQ ID No.3所示；A2、制备G6H1品系的DNA模板稀释液；A3、配制定量PCR反应体系；A4、进行定量PCR检测。本发明的方法具有高效、灵敏、准确等优点，适合于转基因抗虫耐除草剂水稻G6H1及其制品的定量检测。
外文摘要：   NOVELTY - Method of quantitatively PCR detecting transgenic rice G6H1 strain involves (a) designing synthetic primer and fluorescent probe, where the sequences of the primer comprises SEQ ID NO: 1 and SEQ ID NO: 2 and the sequence of the fluorescent probe comprises SEQ ID NO: 3, (b) preparing diluted DNA template solution of the G6H1 strain, and (c) preparing quantitative PCR reaction system and performing quantitative PCR detection.    USE - The method is useful for quantitatively PCR detecting transgenic rice G6H1 strain.    ADVANTAGE - The method has high sensitivity and accuracy and is suitable for the quantitative detection of the transgenic insect-resistant rice G7H1 and its product.    DETAILED DESCRIPTION - Method of quantitatively PCR detecting transgenic rice G6H1 strain involves (a) designing synthetic primer and fluorescent probe, where the sequences of the primer comprises SEQ ID NO: 1 and SEQ ID NO: 2 and the sequence of the fluorescent probe comprises SEQ ID NO: 3, (b) preparing diluted DNA template solution of the G6H1 strain, and (c) preparing quantitative PCR reaction system and performing quantitative PCR detection. 5'-aagcgtcaatttgtttacacc-3' (SEQ ID NO: 1), 5'-tcgatctgctgcagcttg-3' (SEQ ID NO: 2), and 5'-FAM (RTM: 5-carboxyfluorescein)-atggatgtatcgccaccagcacc-Black hole quencher-1 (RTM: Non-fluorescent chromophore)-3' (SEQ ID NO: 3). 
主权项：一种转基因水稻G6H1品系特异性定量PCR检测方法，其特征在于，包括以下步骤： A1、设计合成引物和荧光探针；所述引物的序列如SEQ ID No.1和SEQ ID No.2所示，所述荧光探针的序列如SEQ ID No.3所示； A2、制备G6H1品系的DNA模板稀释液； A3、配制定量PCR反应体系； A4、进行定量PCR检测。
申请号：CN201711031096.8
专利号：CN107858444A
公开/公告号：CN107858444A
申请/专利权人：上海交通大学;
发明/设计人：张大兵; 石建新; 李荣; 杨立桃
分类号： C12Q1/6895; C12Q1/686;
主分类号：C12Q1/6895
法律状态：实质审查
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