中文摘要：本发明提供了引物对和探针组合，本发明还提供了所述的引物对和探针组合在同时检测或辅助检测转基因大豆DAS81419及大豆内源基因中的应用；本发明同时还提供了含有该引物对和探针组合的试剂盒及应用，以及基于引物对和探针组合或试剂盒的检测转基因大豆DAS81419及大豆内源基因的方法。经实验证明，本发明的引物对和探针组合特异性好、灵敏度高、检测效率高，且基于该引物对和探针组合建立的转基因大豆DAS81419和大豆内源基因的双重荧光PCR方法高通量、灵敏、准确和快速，为同时检测转基因大豆DAS81419提供了更有效的方法。
外文摘要：   NOVELTY - Primer pair and probe combination comprises first set of primer pair, second set of primer pair, first probe and second probe.    USE - The primer pair and probe combination is useful in kit for detecting or assisting detection of transgenic soybean DAS81419 and soybean endogenous genes, or preparing product for detecting or assisting detection of transgenic soybean DAS81419 and soybean endogenous genes (all claimed).    ADVANTAGE - The kit detects transgenic soybean DAS81419 and soybean endogenous genes rapidly and accurately with high sensitivity and specificity.    DETAILED DESCRIPTION - Primer pair and probe combination comprises first set of primer pair, second set of primer pair, first probe and second probe. The first set of primer pair comprises base pair sequences of SEQ ID NOs: 1 and 2. The first probe corresponds to the first set of primer pair. The first probe comprises base pair sequence of SEQ ID NO: 3. The second set of primer pair comprises base pair sequences of SEQ ID NOs: 4 and 5. The second probe corresponds to the second set of primer pair. The second probe comprises base pair sequence of SEQ ID NO: 6. agcacttattggtacttgtccta (SEQ ID NO: 1), gtcaaacatgctaagcgact (SEQ ID NO: 2), atcttgaagcaagttccgctca (SEQ ID NO: 3), ccagcttcgccgcttccttc (SEQ ID NO: 4), gaaggcaagcccatctgcaagcc (SEQ ID NO: 5), and cttcaccttctatgcccctgacac (SEQ ID NO: 6). INDEPENDENT CLAIMS are included for the following:    (1) kit comprising the above-mentioned primer pair and probe combination; and    (2) method for detecting or assisting detection of transgenic soybean DAS81419 and soybean endogenous genes by fluorescence PCR, involves (a) performing fluorescence PCR amplification using biological sample containing DNA as a template and primer pair and probe combination or the above-mentioned kit, and (b) determining whether the biological sample is transgenic soybean DAS81419, or biological sample containing soybean components, or biological sample containing transgenic soybean DAS81419 and/or lineage-specific genetic components of the soybean component based on the results of the fluorescent PCR amplification.
主权项：引物对和探针组合，其特征在于，所述引物对和探针组合包括第一组引物对、第二组引物对、第一探针和第二探针，所述第一组引物对包含SEQ ID NO.1和SEQ ID NO.2所示的核苷酸序列，所述第一探针与所述第一组引物对相对应，所述第一探针包含SEQ ID NO.3所示的核苷酸序列；所述第二组引物对包含SEQ ID NO.4和SEQ ID NO.5所示的核苷酸序列，所述第二探针与所述第二组引物对相对应，所述第二探针包含SEQ ID NO.6所示的核苷酸序列。
申请号：CN201610834257.6
专利号：CN107868838A
公开/公告号：CN107868838A
申请/专利权人：湖北出入境检验检疫局检验检疫技术中心;
发明/设计人：赵晖; 乾义柯; 魏霜; 郑剑
分类号：C12Q1/6895; C12Q1/686; C12N15/11;
主分类号：C12Q1/6895
法律状态：实质审查
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