中文摘要：本发明公开植物转基因鉴别装置及方法，包括发泡载板，所述发泡载板上表面和下表面均设置有指槽，所述发泡载板上表面设置有嵌入槽，所述嵌入槽内设置有正电荷薄膜，所述嵌入槽的槽壁上镶嵌有连接链母链，所述正电荷薄膜的边沿上均设置有翻边形成槽状，所述翻边上均设置有与连接链母链相配对的连接链子链，所述正电荷薄膜上设置有储物槽，所述储物槽呈矩形阵列分布，所述储物槽内设置有外源基因探针，所述发泡载板上设置有用于盖住嵌入槽的护罩，所述护罩为透明设置，所述护罩上设置有插入部；该鉴别植物转基因和非转基因的装置可以对多种转基因的外源性基因作出快速、准确和定性的判断分析，可以有效的满足市场的需求。
外文摘要：   NOVELTY - Plant transgenic identification device comprises a foaming carrier board whose both upper surface and the lower surface are provided with finger grooves. An embedding groove is arranged on the surface of the foamed carrier board. A positive charge film is disposed in the embedding groove. The groove of the embedded groove is inlaid with a connecting chain parent chain. The edge of the positive charge film is provided with a flanging groove shape. Each of the flanges is provided with a connection chain which is paired with a link chain. The positive charge film is provided with a storage tank.    USE - The device is useful in detecting genetically modified plants (claimed).    ADVANTAGE - The device can rapidly identify transgenic and non-transgenic plants, has accurate and qualitative judgment analysis, and can effectively meets the requirements of the market.    DETAILED DESCRIPTION - Plant transgenic identification device comprises a foaming carrier board whose both upper surface and the lower surface are provided with finger grooves. An embedding groove is arranged on the surface of the foamed carrier board. A positive charge film is disposed in the embedding groove. The groove of the embedded groove is inlaid with a connecting chain parent chain. The edge of the positive charge film is provided with a flanging groove shape. Each of the flanges is provided with a connection chain which is paired with a link chain. The positive charge film is provided with a storage tank. The storage tanks are distributed in a rectangular array. The exogenous gene probe is set in the storage tank. The foaming carrier is provided with a cover for covering the embedded groove. The shield is transparent. The cover is provided with an insertion portion. The foamed carrier plate is provided with an insertion hole that fits into the insertion portion. The insertion hole is a through hole. The insertion portion is inserted into the insertion hole. The foaming carrier and the shield are detachably connected through the insertion portion and the insertion hole. The foamed carrier is composed of 15-17 pts. wt. green silicon carbide, 44-48 pts. wt. carbon fiber yarns, 57-75 pts. wt. glass fiber filaments, 5-11 pts. wt. hollow glass beads, 15-20 pts. wt. copper fiber, 18-28 pts. wt. terpene resin, 90-133 pts. wt. rosin, 44-53 pts. wt. coba resin, 1-4 pts. wt. sodium stearate, 1-3 pts. wt. tartaric acid, 12-15 pts. wt. fumed silica, 19-23 pts. wt. Arabic gum and 6-9 pts. wt. sodium bicarbonate. The connection chain parent chain and the connection chain chain are PE double-denier sealed chain parent chain and PE double-denier sealed chain chain, respectively. The exogenous gene probe is a 35S promoter, FMV35S promoter, Nos terminator NPTII gene, CryI(AC) gene, Cry9c gene, modified CP4-EPSPS gene, 35s-CTP2, CP4-EPSPS gene, GOX gene, Bar gene, Barnase gene, Barstar gene or CryIA(a) gene. An INDEPENDENT CLAIM is also included for detecting genetically modified plants, comprising (a) preparing exogenous gene probe by placing the raw materials mentioned above into a processing point into the storage tank; (b) using 5 mu g polymerase chain reaction amplification product for denaturation at 100 degrees C in pure water for 5 minutes, cooling directly in ice for 3-5 minutes, using obtained pre-hybridization solution to hybridization of exogenous gene probes, wetting the exogenous gene probes with 0.25 M phosphate buffer, adding the pre-hybridization solution to the exogenous gene probe and pre-hybridizing at 60 degrees C for 10-12 hours, washing with washing solution twice, each time for 20 minutes, dyeing for 5-10 minutes, rinsing with TE buffer for 5-10 minutes, repeating the process twice, detecting signal at 254 nm under UV or fluorescence microscope, and then concluding the results.    DESCRIPTION OF DRAWING(S) - The drawing shows a schematic representation of the plant transgenic identification device. 
主权项：植物转基因鉴别装置，其特征在于：包括发泡载板，所述发泡载板上表面和下表面均设置有指槽，所述发泡载板上表面设置有嵌入槽，所述嵌入槽内设置有正电荷薄膜，所述嵌入槽的槽壁上镶嵌有连接链母链，所述正电荷薄膜的边沿上均设置有翻边形成槽状，所述翻边上均设置有与连接链母链相配对的连接链子链，所述正电荷薄膜上设置有储物槽，所述储物槽呈矩形阵列分布，所述储物槽内设置有外源基因探针，所述发泡载板上设置有用于盖住嵌入槽的护罩，所述护罩为透明设置，所述护罩上设置有插入部，所述发泡载板上设置有与插入部相契合的插入孔，所述插入孔为通孔，所述插入部插入插入孔内，所述发泡载板和护罩通过插入部和插入孔可拆卸连接，所述发泡载板由按重量份数配比的绿碳化硅15-17份、碳纤维丝44-48份、玻璃纤维丝57-75份、空心玻璃微珠5-11份、铜纤维15-20份、萜烯树脂18-28份、松香90-133份、柯巴树脂44-53份、硬脂酸钠1-4份、酒石酸1-3份、气相白炭黑13-15份、阿拉伯胶19-23份和碳酸氢钠6-9份组成,所述连接链母链和连接链子链分别为PE双牙密封链母链和PE双牙密封链子链,所述外源基因探针为35S启动子或FMV35S启动子或Nos终止子或NPTII基因或CryI(AC)基因或Cry9c基因或改造的CP4-EPSPS基因或35s-CTP2或CP4-EPSPS基因或GOX基因或Bar基因或Barnase基因或Barstar基因或CryIA(a)基因中的一种或多种。
申请号：CN201711099077.9
专利号：CN107904157A
公开/公告号：CN107904157A
申请/专利权人：江苏嘉特朗达环保科技有限公司;
发明/设计人：樊璠
分类号：C12M1/34; C12M1/00; C12Q1/6816;
主分类号：C12M1/34
法律状态：实质审查
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