NOVELTY - Inhibiting the growth of a target pathogen expressing the cysteine-rich secreted protein (CSP) comprises contacting the target pathogen with an inhibitor against the CSP, where the inhibitor inhibits the CSP expression and/or binds to a protein product of a gene coding CSP.
USE - The methods are used for inhibiting the growth of a target pathogen expressing the CSP; and controlling aflatoxin-production and/or fungal infection. The inhibitor against the CSP is useful for treating a disease associated with a pathogen expressing CSP and producing aflatoxin, where the disease is selected from acute hepatic necrosis, liver damage, liver cirrhosis, liver cancer, mental impairment, abdominal pain, vomiting, convulsions, edema, pulmonary edema, hemorrhaging and disruption of food digestion, absorption or metabolism (all claimed).
ADVANTAGE - Methods and compounds for a multi-species pathogen control, in particular for aflatoxin and fungal infection control are provided.
DETAILED DESCRIPTION - INDEPENDENT CLAIMS are included for:
(1) an isolated polynucleotide selected from (i) a polynucleotide derived from a nucleic acid sequence selected from SEQ ID NO: 1 or 2; (ii) a polynucleotide comprising a nucleic acid sequence selected from SEQ ID NO: 1 or 2; (iii) a polynucleotide that hybridizes to a nucleic acid sequence selected from SEQ ID NO: 1 or 2 under stringent conditions; (iv) a polynucleotide of 70-90% sequence identity, to a nucleic acid sequence selected from SEQ ID NO: 1 or 2; (v) a fragment of greater than or equal to 16 contiguous nucleotides of a nucleic acid sequence selected from SEQ ID NO: 1 or 2; and (vi) a complement of the sequence of (i)-(v);
(2) a plant transformed, transduced or transfected with a polynucleotide of (1);
(3) a method for controlling aflatoxin-production and/or fungal infection by providing an agent comprising a first polynucleotide sequence that functions upon uptake by the fungi to inhibit a biological function within the fungi, where the polynucleotide sequence exhibits 95-100% nucleotide sequence identity along 16-30 contiguous nucleotides to a CSP coding sequence derived from the fungi and is hybridized to a second polynucleotide sequence that is complementary to the first polynucleotide sequence;
(4) a small interfering RNA (siRNA) for inhibiting the expression of a CSP protein in a target pathogen, where the siRNA comprises greater than or equal to 2 sequences that are complementary to each other and where a sense strand comprises a first sequence and an anti-sense strand comprises a second sequence comprising a region of complementarity, which is substantially complementary to a part of an mRNA encoding a nucleotide sequence from SEQ ID NO: 1; and
(5) a method of treating a disease associated with a pathogen by administering an inhibitor against the CSP to a patient.申请号：WO2016165729-A1
专利号：WO2016165729-A1
公开/公告号：WOEP057943
申请/专利权人：FRAUNHOFER GES FOERDERUNG ANGEWANDTEN EV (FRAU-C)
发明/设计人：NOLKE, G；SCHILLBERG, S；SCHUBERT, M
分类号：C07K-014/38; C12N-015/82
点击下载：用抗富含半胱氨酸分沁蛋白（CSP）的抑制剂来抑制表达CSP的目标病原菌的生长