中文摘要：研究以恒河猴为对象比较了腺病毒介导的疫苗通过肠道免疫、小剂量喷雾和大剂量喷雾免疫对细胞内和体液免疫的影响，分析不同免疫方式下的原发性免疫反应和对已有的抗原接触性系统免疫反应的触发情况。喷雾免疫激发产生的肺部CD4和CD8 T-cell反应最高，在支气管肺泡灌洗液中有10-30%的细胞为疫苗特异性细胞。相对而言，肠道免疫的T细胞反应比喷雾免疫低10倍以上。只有4 pm剂量的喷雾免疫组和肠道免疫组激发了系统（血液）T细胞反应，所有免疫组的体液免疫反应均较弱。这些结果表明，不同的呼吸道免疫方式会产生不同的免疫反应，且小剂量喷雾免疫的效果优于其它免疫方式。
外文摘要：Respiratory mucosa immunization is capable of eliciting both local and distal mucosal immune responses; it is a potentially powerful yet largely unused modality for vaccination against respiratory diseases. Targeting the lower versus upper airways by aerosol delivery alters the immunogenicity profile of a vaccine, although the full extent of this impact is not well characterized. We set out to define the cellular and humoral response profiles elicited by immunization via intranasal, small aerosol droplets, and large aerosol droplets. We compared responses following adenovirus-vectored vaccination by these routes in macaques, either for the generation of primary immune responses or for the boosting of previously primed systemic responses. Aerosol delivery (4 or 10 mu m diameter droplets, addressing lower or upper airways, respectively) generated the highest magnitude lung CD4 and CD8 T-cell responses, reaching 10-30% vaccine-specific levels in bronchoalveolar lavage cells. In contrast, intranasal delivery was less immunogenic with >10-fold lower peak lung T-cell responses. Systemic (blood) T-cell responses were only observed following 4 pm aerosol (and parenteral) immunization, while all delivery routes elicited similar humoral responses. These data demonstrate distinct immune response profiles with each respiratory tract vaccination modality and suggest that small droplet aerosol offers several immunological advantages over other respiratory routes.
外文关键词：Aerosol；Nasal；Vaccine delivery route；Immunogenicity；Rhesus macaque
作者：Bolton, DL; Song, KM; Tomaras, GD; Rao, S; Roederer, M
作者单位：美国感染与免疫研究所
期刊名称：VACCINE
期刊影响因子：3.413
出版年份：2017
出版刊次：1
点击下载：喷雾、鼻腔和肠道免疫对恒河猴细胞内和体液免疫原性的影响分析