中文摘要：本研究表明，一种具有免疫刺激作用的细菌性蛋白酶抑制剂U-Omp19能够通过口服与沙门氏菌相互作用，从而增强小鼠的粘膜和系统免疫反应。U-Omp19能够提高抗原特异性细胞因子IFN-γ和IL-17的水平，增强粘膜抗体（IgA）反应。将U-Omp19和沙门氏菌抗原一起口服能够在鼠伤寒沙门氏杆菌感染情况下刺激机体产生保护作用，显著降低细菌感染对机体的反应。这些研究证明，U-Omp19是一种合适的口服疫苗的佐剂。
外文摘要：Most pathogens infect through mucosal surfaces, and parenteral immunization typically fails to induce effective immune responses at these sites. Development of oral-administered vaccines capable of inducing mucosal as well as systemic immunity while bypassing the issues of antigen degradation and immune tolerance could be crucial for the control of enteropathogens. This study demonstrates that U-Omp19, a bacterial protease inhibitor with immunostimulatory features, coadministered with Salmonella antigens by the oral route, enhances mucosal and systemic immune responses in mice. U-Omp19 was able to increase antigen-specific production of IFN-gamma and IL-17and mucosal (IgA) antibody response. Finally, oral vaccination with U-Omp19 plus Salmonella antigens conferred protection against virulent challenge with Salmonella Typhimurium, with a significant reduction in bacterial loads. These findings prove the efficacy of this novel adjuvant in the Salmonella infection model and support the potential of U-Omp19 as a suitable adjuvant in oral vaccine formulations against mucosal pathogens requiring T helper (Th) 1-Th17 protective immune responses.
外文关键词：adjuvant；vaccination；Salmonella Typhimurium；T helper 1；T helper 17
作者：Risso, GS; Carabajal, MV; Bruno, LA; Ibanez, AE; Coria, LM; Pasquevich, KA; Lee, SJ; McSorley, SJ; Briones, G; Cassataro, J
作者单位：阿根廷 Univ Nacl San Martin UNSAM
期刊名称：FRONTIERS IN IMMUNOLOGY
期刊影响因子：5.695
出版年份：2017
出版刊次：2
点击下载：源于牛布氏杆菌的U-Omp19是小鼠抗沙门氏菌感染疫苗的有效佐剂