中文摘要：本研究评估了纳米脂质体佐剂增强抗痢疾阿米巴 Gal/Ga1NAc血凝素LecA抗原粘膜免疫反应的能力。分别以包含TLR抗原的明矾、乳剂和脂质体对CBA/J小鼠进行免疫。结果表明：包含TLR抗原的纳米脂质体传递系统可用于抗肠道病原菌疫苗的制备，尤其适用于激发多种免疫反应的情况。
外文摘要：Diarrheal infectious diseases represent a major cause of global morbidity and mortality. There is an urgent need for vaccines against diarrheal pathogens, especially parasites. Modern subunit vaccines rely on combining a highly purified antigen with an adjuvant to increase their efficacy. In the present study, we evaluated the ability of a nanoliposome adjuvant system to trigger a strong mucosal immune response to the Entamoeba histolytica Gal/Ga1NAc lectin LecA antigen. CBA/J mice were immunized with alum, emulsion or liposome based formulations containing synthetic TLR agonists. A liposome formulation containing TLR4 and TLR7/8 agonists was selected based on its ability to generate intestinal IgA, plasma IgG2a/IgG1, IFN-gamma, and IL-17A. Immunization with a mucosal prime followed by a parenteral boost generated a high mucosal IgA response that inhibited adherence of parasites to mammalian cells. Inclusion of the immune potentiator all-trans retinoic acid in the regimen further improved the mucosal IgA response. Immunization protected from infection with up to 55% efficacy. Our results show that a nanoliposome delivery system containing TLR agonists is a promising prospect for the development of vaccines against enteric pathogens, especially when a multifaceted immune response is desired.
外文关键词： Liposome; Vaccine; Immune response; Amebiasis; Entamoeba
作者：Abhyankar, MM; Noor, Z; Tomai, MA; Elyecrog, J; Fox, CB; Petri, WA
作者单位：美国弗吉尼亚大学
期刊名称：VACCINE
期刊影响因子：3.413
出版年份：2017
出版刊次：2
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