中文摘要：研究分析了PCEP和鸡β-防御素（ABD）作为佐剂包含在鸡卵黄疫苗中的活性。结果表明：PCEP与灭活病毒结合能够诱导产生强烈的、持续的抗体反应；在PCEP的作用下，IFN-γ、IFN-α、IL-12（p40）和IL-5的基因表达水平显著增强；这些结果表明，卵黄疫苗佐剂PCEP能够激发鸡产生大量的平衡的免疫反应。
外文摘要： Inclusion body hepatitis (IBH) is one of the major viral infections causing substantial economic loss to the global poultry industry. The disease is characterized by a sudden onset of mortality (2-30%) and high morbidity (60-70%). IBH is caused by a number of serotypes of fowl adenovirus with substantially low levels of serotype cross protection. Thus far, there is no effective and safe vaccine commercially available in the North America for the control of IBH in chickens. Poly[di(sodium carboxylatoethylphenoxy)]phosphazene (PCEP) is a high molecular weight, biodegradable water soluble polymer that has been well characterized as a safe and effective adjuvant for a number of experimental veterinary vaccines. Similarly, host defence peptides, including beta-defensins, have also been shown to exhibit strong adjuvant potential. In this study, we evaluated the adjuvant activity of PCEP and avian beta defensin (ABD) in a vaccine formulation containing inactivated fowl adenovirus (FAdV) serotype 8b administered in ovo. Our data showed that a combination of PCEP and inactivated virus is capable of inducing a robust and long lasting antibody response. Moreover, significant enhancement of IFN-gamma,IFN-alpha, IL-12(p40) and IL-6 gene expression under the influence of PCEP suggests that as an in ovo adjuvant PCEP has the ability to activate a substantial balanced immune response in chickens. To our knowledge, these are the first studies in which PCEP and ABD have been characterized as adjuvants for the development of an in ovo poultry vaccine. It is expected that these preliminary studies will be helpful in the development of safer and more effective in ovo vaccine against IBH and other infectious diseases affecting chickens.
外文关键词： Fowl adenovirus serotype 8b; Inclusion body hepatitis; Broilers; In ovo vaccines; Adjuvants; PCEP; Avian beta defensin; Humoral immunity; Cellular immunity
作者：Sarfraz, M; Suleman, M; Tikoo, SK; Wheler, C; Potter, AA; Gerdts, V; Dar, A
作者单位：萨斯喀彻温省大学
期刊名称：VACCINE
期刊影响因子：3.413
出版年份：2017
出版刊次：2
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