中文摘要：研究探索了应用宿主干扰素诱导Mx蛋白来提高HA2 H9N2流感DNA疫苗的免疫原性。应用BALB/c小鼠评估以Mx为佐剂的HA2疫苗的效力和安全性。研究结果表明，Mx作为宿主防御肽能够提供疫苗的效力。
外文摘要：Immunization with DNA vaccines as a novel alternative to conventional vaccination strategy requires adjuvant for improving vaccine efficacy. The conserved immunogenic HA2 subunit, which harbors neutralizing epitopes is a promising vaccine candidate against influenza viruses. In this study, for the first time we explore the idea of using host interferon inducible Mx protein to increase the immunogenicity of HA2 H9N2 influenza DNA vaccine. The potency and safety of the Mx adjuvanted-HA2 vaccine was evaluated in BALB/c mice by different prime-boost strategies. To assess the effect of the vaccination on the virus clearance rate, mice were challenged with homologous influenza virus. Administration of the adjuvanted vaccine and boosting with the same regimen could effectively enhance both humoral and cellular immune responses in treated mice. These data demonstrated that Mx as host defense peptide can be potentiated for improving influenza vaccine efficacy.
外文关键词： Influenza; Immune responses; HA2 subunit; Mx; Bio adjuvant
作者：Soleimani, S; Shahsavandi, S; Maddadgar, O
作者单位：伊朗拉齐疫苗和血清研究所
期刊名称：BIOLOGICALS
期刊影响因子：1.69
出版年份：2017
出版刊次：3
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