中文摘要：作者通过先前的研究证实，乳酸乳球菌纤连蛋白结合蛋白A（FnBPA+）(pValac: ESAT-6)作为活粘膜DNA疫苗通过口腔免疫小鼠可以刺激产生特异性免疫反应。本文鉴定了可促进小鼠卡介苗疫苗免疫反应的乳酸乳球菌类型，结果证实，L. lactis FnBPA+ (pValac: ESAT-6)能够提高卡介苗疫苗一般免疫反应，可作为卡介苗疫苗的传递系统应用。
 
外文摘要：Aims: A regimen utilizing Bacille Calmette-Guerin (BCG) and another vaccine system as a booster may represent a promising strategy for the development of an efficient tuberculosis vaccine for adults. In a previous work, we confirmed the ability of Lactococcus lactis fibronectin-binding protein A (FnBPA+) (pValac: ESAT-6), a live mucosal DNA vaccine, to produce a specific immune response in mice after oral immunization. In this study, we examined the immunogenicity of this strain as a booster for the BCG vaccine in mice. Methods and Results: After immunization, cytokine and immunoglobulin profiles were measured. The BCG prime L. lactis FnBPA+ (pValac: ESAT-6) boost group was the most responsive group, with a significant increase in splenic pro-inflammatory cytokines IL-17, IFN-gamma, IL-6 and TNF-alpha compared with the negative control. Conclusions: Based on the results obtained here, we demonstrated that L. lactis FnBPA+ (pValac: ESAT-6) was able to increase the BCG vaccine general immune response. Significance and Impact of the Study: This work is of great scientific and social importance because it represents the first step towards the development of a booster to the BCG vaccine using L. lactis as a DNA delivery system.
外文关键词：BCG; DNA vaccine; ESAT-6; Lactococcus lactis; tuberculosis
作者：Pereira, VB; Da Cunha, VP; Preisser, TM; Souza, BM; Turk, MZ; De Castro, CP; Azevedo, MSP; Miyoshi, A
作者单位：巴西米纳斯联邦大学
期刊名称： JOURNAL OF APPLIED MICROBIOLOGY
期刊影响因子：2.156
出版年份：2017
出版刊次：6
点击下载：一种携带DNA疫苗的乳酸乳球菌能够增加IL-17细胞因子分泌、促进卡介苗疫苗的免疫反应