中文摘要：研究分析了胚芽乳酸菌在粘膜免疫中作为沙眼衣原体抗原传递载体的作用。结果表明，重组胚芽乳酸菌作为免疫促进剂增强细胞内免疫反应。应用包含胚芽乳酸菌的疫苗进行促进免疫可以诱导鞘膜腔内抗原特异性IgA的产生。
 
外文摘要：Mucosal immunity is important for the protection against a wide variety of pathogens. Traditional vaccines administered via parenteral routes induce strong systemic immunity, but they often fail to generate mucosal IgA. In contrast, bacteria-based vaccines comprise an appealing strategy for antigen delivery to mucosal sites. Vaginal infection with Chlamydia trachomatis can develop into upper genital tract infections that can lead to infertility. Therefore, the development of an effective vaccine against Chlamydia is a high priority. In the present study, we have explored the use of a common lactic acid bacterium, Lactobacillus plantarum, as a vector for delivery of a C. trachomatis antigen to mucosal sites. The antigen, referred as Hirep2 (H2), was anchored to the surface of L. plantarum cells using an N-terminal lipoprotein anchor. After characterization, the constructed strain was used as an immunogenic agent in mice. We explored a heterologous prime-boost strategy, consisting of subcutaneous priming with soluble H2 antigen co-administered with CAF01 adjuvant, followed by an intranasal boost with H2-displaying L. plantarum. The results show that, when used as a booster, the recombinant L. plantarum strain was able to evoke cellular responses. Most importantly, booster immunization with the Lactobacillus-based vaccine induced generation of antigen-specific IgA in the vaginal cavity.
 
外文关键词：LACTIC-ACID BACTERIA; TOXIN FRAGMENT-C; IMMUNE-RESPONSES; TETANUS TOXIN; DENDRITIC CELLS; SUBUNIT VACCINES; T-CELLS; INFECTION; VACCINATION; PROTECTION
作者：Kuczkowska, K; Myrbraten, I; Overland, L; Eijsink, VGH; Follmann, F; Mathiesen, G; Dietrich, J
作者单位：挪威生命科学大学
期刊名称：PLOS ONE
期刊影响因子：3.057
出版年份：2017
出版刊次：5
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