中文摘要：研究构建了一个编码刚地弓形虫致密颗粒14（GRA14）的质粒，分析了磷酸钙纳米颗粒（CaPNs）作为纳米佐剂在增强对pcGRA14保护效率方面的作用。结果表明：小鼠应用pcGRA14 DNA疫苗能够诱导免疫反应。纳米颗粒包裹的pcGRA14能够显著增强机体抗弓形虫感染的细胞反应和机体反应。
 
外文摘要：Several approaches have been used to improve the immunogenicity of DNA vaccines. In the current study, we constructed the plasmid encoding T. gondii dense granule 14 (GRA14) and investigated the immunological properties of calcium phosphate nanoparticles (CaPNs) as nano-adjuvant to enhance the protective efficacy of pcGRA14. BALB/c mice intramuscularly injected three times at two-week intervals and the immune responses were evaluated using lymphocyte proliferation assay, cytokine and antibody measurements, survival times, and parasite load of mice challenged with the virulent T. gondii RH strain. The results showed that the immune responses were induced in mice receiving pcGRA14 DNA vaccine. Interestingly, pcGRA14 coated with nanoparticles led to statistically significant enhancements of cellular and humoral immune responses against Toxoplasma infection (P < 0.05). After challenge with RH strain of T. gondii, immunized mice with pcGRA14 showed prolong survival time compared to control groups (P < 0.05). In addition, pcGRA14 coated with nano-adjuvant exhibited the lowest parasitic load in the infected mice tissues. For the first time, our data indicate that the pcGRA14 coated with CaPN was more effective for stimulation of immune responses and should be considered as an adjuvant in the design of vaccines against toxoplasmosis.
外文关键词：Toxoplasma gondii; DNA vaccine; Calcium phosphate nanoparticles; Nano-adjuvant; GRA14 gene; Dense granule; Immune response
作者：Ahmadpour, E; Sarvi, S; Soteh, MBH; Sharif, M; Rahimi, MT; Valadan, R; Tehrani, M; Khalilian, A; Montazeri, M; Fasihi-Ramandi, M; Daryani, A
作者单位：伊朗马赞达兰大学
期刊名称：IMMUNOLOGY LETTERS
期刊影响因子：2.483
出版年份：2017
出版刊次：5
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