中文摘要：本研究构建了一种新型疫苗GamTBvac，该疫苗由两种分歧杆菌抗原融合蛋白组成，两种融合蛋白采用右旋糖酐固定，并与包含DEAE-右旋糖酐核和CpG脱氧寡核苷酸（TLR9促进剂）构成的佐剂混合。分别以小鼠和豚鼠模型评价了GamTBvac疫苗/促进剂、BCG疫苗/GamTBva促进剂的免疫原性和保护效率。结果表明：GamTBvac具有较强的免疫原性，采用喷雾或静脉注射时，对抑制结核分歧杆菌H37Rv具有显著的保护作用，作为一种BCG促进疫苗能够表现出较强的保护作用。
外文摘要：New innovative vaccines are highly needed to combat the global threat posed by tuberculosis. Efficient components-antigens and adjuvants-are crucial for development of modern recombinant TB vaccines. This study describes a new vaccine (GamTBvac) consisting of two mycobacterial antigen fusions (Ag85A and ESAT6-CFP10)-with dextran-binding domain immobilized on dextran and mixed with an adjuvant consisting of DEAE-dextran core, and with CpG oligodeoxynucleotides (TLR9 agonists). GamTBvac and its components were assessed for immunogenicity and protective efficacy in GamTBvac-prime/boost and BCG-prime/GamTBvac-boost in murine and guinea pig TB models. Results show that in both infectious models, GamTBvac has a strong immunogenicity and significant protective effect against Mycobacterium tuberculosis strain H37Rv under aerosol and intravenous challenges. GamTBvac showed a particularly strong protective effect as a BCG booster vaccine.
外文关键词：BACILLUS-CALMETTE-GUERIN; TUBERCULOSIS VACCINE; INFECTION; PROSPECTS; RESPONSES; DURATION; IMMUNITY; FAILURE; DEXTRAN; TRIAL
作者：Tkachuk, AP; Gushchin, VA; Potapov, VD; Demidenko, AV; Lunin, VG; Gintsburg, AL
作者单位：NF Gamaleya联邦流行病学和微生物学研究中心
期刊名称：PLOS ONE
期刊影响因子：2.806
出版年份：2017
出版刊次：4
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