中文摘要：AS03佐剂是一种α-麦胚酚水包油乳剂佐剂系统，本研究分析了AS03佐剂在A(H1N1)pdm09流感疫苗体液和细胞调节免疫反应持续性方面的作用。结果表明：AS03佐剂能够增强A(H1N1)pdm09流感疫苗体液和细胞调节免疫反应的持续性，从而降低抗原的使用量，减缓年龄和前期季节性免疫的副作用。
 
外文摘要：We investigated the role of AS03(A) (here AS03), an alpha-tocopherol oil- in-water emulsion-based adjuvant system, on the long-term persistence of humoral and cell-mediated immune responses to A(H1N1) pdm09 influenza vaccines. In two studies, a total of 261 healthy adults (<= 60 years old) were randomized to receive two doses of AS03-adjuvanted vaccine containing 3.75 mu g of hemagglutinin (HA) or nonadjuvanted vaccine containing 15 mu g of hemagglutinin (in study A) or 3.75 mu g of hemagglutinin (in study B) 21 days apart. Hemagglutination inhibition (HI) antibody, memory B-cell, and CD4(+)/CD8(+) T-cell responses were characterized up to 1 year following dose 1. We also assessed the effects of age and seasonal influenza vaccination history. AS03-adjuvanted (3.75 mu g HA) vaccine and nonadjuvanted vaccine at 15 mu g but not at 3.75 mu g HA elicited HI antibody responses persisting at levels that continued to meet European licensure criteria through month 12. At month 12, the geometric mean titer for AS03-adjuvanted vaccine was similar to that for nonadjuvanted (15-mu g) vaccine in study A (1: 86 and 1: 88, respectively) and higher than that for nonadjuvanted (3.75-mu g) vaccine in study B (1: 77 and 1: 35, respectively). A(H1N1) pdm09-specific CD4(+) T-cell and B-cell responses were stronger in AS03-adjuvanted groups and persisted only in these groups for 12 months at levels exceeding prevaccination frequencies. Advancing age and a seasonal vaccination history tended to reduce HI antibody and memory B-cell responses and, albeit less consistently, CD4(+) T-cell responses. Thus, AS03 seemed to enhance the persistence of humoral and cell-mediated responses to A(H1N1) pdm09 vaccine, allowing for antigen sparing and mitigating potential negative effects of age and previous seasonal vaccination.
外文关键词：AS03 adjuvant； influenza vaccine； pandemic influenza
作者： van der Most, RG; Clement, F; Willekens, J; Dewe, W; Walravens, K; Vaughn, DW; Leroux-Roels, G
作者单位：比利时葛兰素疫苗事业部
期刊名称：CLINICAL AND VACCINE IMMUNOLOGY
期刊影响因子：2.425
出版年份：2017
出版刊次：6
点击下载：A（H1N1）pdm09流感疫苗的细胞和体液反应的持续性研究及AS03佐剂系统的作用分析