中文摘要：为寻求一个更加安全有效的疫苗佐剂，研究分析了流感重组血凝素（HA）与一种新型蔬菜油包水乳液佐剂（天然免疫-增强型传递系统，NIDS）混合，该佐剂由维生素A、E和儿茶酚组成，可以提供抗鼻内活流感病毒的保护作用。分别将HA与NIDS、水包三十碳六烯油乳剂、Poly (I: C)等佐剂混合免疫BALB/c小鼠，分析不同佐剂的安全性和免疫效率。结果表明：NIDS表现出明显的剂量节约效应，且能在远系繁殖CD1小鼠体内保持完整的佐剂功效；更为重要的是，采用水包三十碳六烯油乳剂作为佐剂能够诱导产生更高水平的血清淀粉样P成分和更高浓度的总血浆IgE，其中血清淀粉样P成分是肝细胞分泌的一种急性期反应物，而NIDS佐剂不会导致这样的反应。因此NIDS在临床上是一种更安全且更有效的流感疫苗佐剂。
 
外文摘要：Non-replicating vaccines, such as those based on recombinant proteins, require adjuvants and delivery systems, which have thus far depended on mimicking pathogen danger signals and strong pro-inflammatory responses. In search of a safer and more efficacious alternative, we tested whether vaccinations with influenza recombinant hemagglutinin (HA) mixed with a novel vegetable oil in water emulsion adjuvant (Natural Immune-enhancing Delivery System, NIDS), based on the immune-enhancing synergy of vitamins A and E and a catechin, could protect against intra-nasal challenge with live influenza virus. Vaccinations of inbred Brag Albino strain c (BALB/c) mice, with HA mixed with NIDS compared to other adjuvants, i. e., a squalene oil in water emulsion (Sq. oil), and the Toll Like Receptor 3 (TLR3) agonist Poly (I: C), induced significantly lower select innate pro-inflammatory responses in serum, but induced significantly higher adaptive antibody and splenic T Helper 1 (TH1) or TH2, but not TH17, responses. Vaccinations with NIDS protected against infection, as measured by clinical scores, lung viral loads, and serum hemagglutination inhibition titers. The NIDS exhibited a strong dose sparing effect and the adjuvant action of NIDS was intact in the outbred CD1 mice. Importantly, vaccinations with the Sq. oil, but not NIDS, induced a significantly higher Serum Amyloid P component, an acute phase reactant secreted by hepatocytes, and total serum IgE. Thus, the NIDS may be used as a clinically safer and more efficacious vaccine adjuvant against influenza, and potentially other infectious diseases.
外文关键词：vaccine； adjuvant； natural； vitamin A； vitamin E； flavonoid； catechin； influenza； immunity
作者：Patel, S; Faraj, Y; Duso, DK; Reiley, WW; Karlsson, EA; Schultz-Cherry, S; Vajdy, M
作者单位：美国EpitoGenesis 有限公司
期刊名称：NUTRIENTS
期刊影响因子：3.55
出版年份：2017
出版刊次：5
点击下载：一种由维生素A、E和儿茶酚组成的新型水包油疫苗佐剂在抗流感免疫中的安全性和效率研究