中文摘要：研究从比利时、西班牙、英国分离到50个猪肺炎支原体样本，从法国、匈牙利、西班牙、英国分离到150个牛肺炎支原体样本，采用微量肉汤稀释法分析了分离得到的支原体的抗菌敏感性。猪肺炎支原体对恩诺沙星、马波沙星和氟苯尼考的最高MIC90值出现在英国样本中，为1.0 mg/L；相反，牛肺炎支原体的最低MIC90值为1.0 mg/L，最高可达到64 mg/L以上。因此，迫切需要建立起不同动物肺炎支原体特异的实验室检测标准和临床诊断阈值，从而解决体外诊断效率的难题。
外文摘要：Mycoplasma hyopneumoniae in pigs and Mycoplasma bovis in cattle are major pathogens affecting livestock across Europe and are the focus of the MycoPath pan-European antimicrobial susceptibility monitoring programme. Fifty M. hyopneumoniae isolates from Belgium, Spain and the United Kingdom (UK), and 156 M. bovis isolates from France, Hungary, Spain and the UK that met specific criteria were tested for antimicrobial susceptibility in a central laboratory by using a microbroth dilution method. Specific isolate criteria included recovery from animals not recently treated with antimicrobials, isolates from different locations within each country and retaining only one isolate per farm. MIC50/MIC90 values were 0.031/0.5, 0.031/0.5, 0.062/0.25, <= 0.001/0.004, 0.031/0.125, 0.25/0.5 and 0.062/0.25 mg/L for enrofloxacin, marbofloxacin, spiramycin, tulathromycin, tylosin, florfenicol and oxytetracycline respectively against M. hyopneumoniae and 0.25/4, 1/4, 4/16, > 64/ > 64, 32/ > 64, 2/4 and 4/64 mg/L, respectively against M. bovis. MIC50/MIC90 values for tiamulin and valnemulin against M. hyopneumoniae were 0.016/0.062 and <= 0.001/ <= 0.001 mg/L respectively. The MIC50/MIC90 values of danofloxacin and gamithromycin for M. bovis were 0.25/1 and > 64/ > 64 mg/L respectively. The highest MIC90 values for M. hyopneumoniae were found in the UK at 1.0 mg/L for enrofloxacin, marbofloxacin and florfenicol. In contrast, for M. bovis the lowest MIC90 value was 1.0 mg/L, but ranged to > 64 mg/L. Specific laboratory standards and clinical breakpoints for veterinary Mycoplasma species are required as no independently validated clinical breakpoints are specified for veterinary Mycoplasma species, which makes data interpretation and correlation to in vivo efficacy difficult.
外文关键词：Antimicrobial susceptibility； Surveillance； Minimal inhibitory concentrations； Mycoplasma hyopneumoniae； Mycoplasma bovis
作者：Klein, U; de Jong, A; Moyaert, H; El Garch, F; Leon, R; Richard-Mazet, A; Rose, M; Maes, D; Pridmore, A; Thomson, JR; Ayling, RD
作者单位：比利时中东欧学校协会
期刊名称：VETERINARY MICROBIOLOGY
期刊影响因子：2.628
出版年份：2017
出版刊次：5
点击下载：欧洲猪肺炎支原体和牛肺炎支原体的抗菌药物敏感性监测