中文摘要：本研究构建了一种稳定的口蹄疫病毒疫苗水/油混合纳米乳液佐剂；该佐剂以十四烷酸异丙酯作为油相，聚甘油蓖麻醇酯、Tween 80作为表面活性剂。将Tween 80溶解在水相中形成液滴大小，通过缩小尺寸分布提高乳液的稳定性，增加黏性，降低水油界面张力。将该纳米乳液与灭活口蹄疫病毒疫苗、乳清蛋白结合，分析其作为疫苗佐剂的作用。与商业佐剂ISA-206相比，这种新型水/油纳米乳液能够诱导产生体液和细胞免疫反应。
 
外文摘要：A stable and bio-compatible w/o nano-emulsion was developed as adjuvant for foot and mouth disease (FMD) vaccine with isopropyl myristate as oil phase, polyglycerol polyricinoleate, and Tween 80 as surfactants. Dissolving Tween 80 in aqueous phase decreased the droplet size and improved stability of the emulsion through narrowing size distribution, increasing viscosity, and reducing the interfacial tension between oil and water. The nano-emulsion was then formulated with inactivated FMD virus and ovalbumin to investigate its potential application as an adjuvant. Compared with commercial ISA-206 adjuvant, the novel w/o nano-emulsion was able to induce both humoral- and cell-mediated immune response.
外文关键词：Adjuvant； foot-and-mouth disease vaccine； polyglycerol polyricinoleate； stability； w/o nano-emulsion
作者：Chen, ZH; Zhang, SP; Li, ZJ; Ma, GH; Su, ZG
作者单位：中国农业科学院
期刊名称：ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY
期刊影响因子：5.605
出版年份：2017
出版刊次：5
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