中文摘要：本研究构建了一个pcMyD88质粒，并将该质粒作为分子佐剂与编码AcFA的DNA疫苗一起注射，该DNA疫苗能够编码抗斜带石斑鱼溶藻弧菌感染的AcfA蛋白。结果表明，将pcMyD88与pcacfA DNA疫苗一起注射能够显著增加特异性抗体浓度，提高与免疫相关的MHCIα、 MHCIIα、CD4、CD8α、 IL-1 β 和 TNF α等基因的表达量。同时，pcMyD88能够提高pcacfA DNA疫苗的保护作用。这些结果表明，MyD88是斜带石斑鱼pcacfA DNA疫苗的一种有效佐剂。
外文摘要：DNA vaccines had been widely used against microbial infection in animals. The use of molecular adjuvants to improve the immunogenicity of DNA vaccines has been increasingly studied in recent years. MyD88 is one of the adapter molecules to activate the signaling cascades and produces inflammatory mediators, and its immunological role and adjuvant potential which had been proved in mammals were rarely reported in fish species. In this study, plasmid pcMyD88 was constructed and the capacity of MyD88 as molecular adjuvant was explored by co-injecting with a DNA vaccine encoding AcfA against Vibrio alginolyticus infection in orange spotted grouper. The results suggested that it needed at least 7 days to transported DNA vaccine pcacfA or molecular adjuvant pcMyD88 from the injected muscle to kidney and spleens and stimulate host's immune system for later protection. The co-injection of pcMyD88 with DNA vaccine pcacfA could increase significantly specific antibody levels and the expression levels of the immune-related genes including MHCI alpha, MHCII alpha, CD4, CD8 alpha, IL-1 beta and TNF alpha. Furthermore, pcMyD88 enhanced the immunoprotection of pcacfA against V. alginolyticus infection, with the significantly higher RPS of 833% in pcMyD88 pcacfA group compared with that of pcacfA alone (73.3%) at challenging test of 10 weeks post vaccination. Together, these results clearly demonstrate that MyD88 is an effective adjuvant for the DNA vaccine pcacfA in orange spotted grouper.
外文关键词：Vibrio alginolyticus； acfA； MyD88； Molecular adjuvant； DNA vaccine
作者：Huang, YC; Cai, SH; Pang, HY; Jian, JC; Wu, ZH
作者单位：广东海洋大学
期刊名称：FISH & SHELLFISH IMMUNOLOGY
期刊影响因子：3.148
出版年份：2017
出版刊次：7
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