中文摘要：本研究采用单磷酰脂质A（MPL）、TLR4促进剂作为佐剂诱导抗流感嗜血杆菌（NTHi）的粘膜免疫反应，从而加速清除鼻咽部位的细菌。结果表明：MPL能够有效诱导产生抗NTHi的粘膜免疫反应，适合作为粘膜免疫佐剂进行应用。
外文摘要：Objective: Acute otitis media (AOM) is one of the most common infectious diseases in children. Non typeable Haemophilus influenzae (NTHi) is Gram-negative bacteria that are considered major pathogens of AOM and respiratory tract infections. In this study, we used monophosphoryl lipid A (MPL), a toll -like receptor (TLR) 4 agonist, as an adjuvant to induce mucosal immune responses against NTHi to enhance bacterial clearance from the nasopharynx. Methods: Mice were administered 10 jig outer membrane protein (OMP) from NTHi and 0, 10, or 20 mu g MPL intranasally once a week for 3 weeks. Control mice were administered phosphate-buffered saline alone. After immunization, these mice were challenged with NTHi. At 6 and 12 h after bacterial challenge, the mice were killed and nasal washes and sera were collected. The numbers of NTHi- and OMP-specific antibodies were quantified by enzyme-linked immunosorbent assay. Results: The MPL 10 and 20 mu g group produced a significant reduction in the number of bacteria recovered from the nasopharynx at 12 h after bacterial challenge compared to the control group. OMP-specific IgA titers were also augmented in the MPL groups compared to the control and OMP groups. Conclusion: MPL is suitable for eliciting effective mucosal immune responses against NTHi in the nasopharynx. These results demonstrate the possibility of an adjuvant that involves stimulation of the innate immune system by TLR4 agonists such as MPL for mucosal vaccination.
外文关键词： Monophosphoryl lipid A； Nonrypeable Haemophilus influenza； Intranasal immunization
作者：Iwasaki, T; Hirano, T; Kodama, S; Kadowaki, Y; Moriyama, M; Kawano, T; Suzuki, M
作者单位：日本大分大学
期刊名称： INTERNATIONAL JOURNAL OF PEDIATRIC OTORHINOLARYNGOLOGY
期刊影响因子：1.159
出版年份：2017
出版刊次：6
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