N-2-羟丙基二甲基乙基氯化铵壳聚糖纳米颗粒包裹的活新城疫疫苗的免疫反应分析

Response of live Newcastle disease virus encapsulated in N-2-hydroxypropyl dimethylethyl ammonium chloride chitosan nanoparticles

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中文摘要:研究合成了一种N-2-羟丙基二甲基乙基氯化铵壳聚糖(N-2-HFCC),其水溶性优于几丁聚糖。为评价N-2-HFCC作为传递载体的性能,研究将新城疫活疫苗包裹进N-2-HFCC/CMC纳米颗粒中,其大小为252.2±32.68 nm,ZETA电位41.1±0.89mV,包封率96.6±0.72%,载荷能力53.2±1.11%。NDV/La Sota-N-2-HFCC/CMC-NPs是一种新型的包裹在纳米颗粒中的灭活疫苗。这些纳米颗粒细胞毒性更低、稳定性更好。体外试验表明,应用NDV/La Sota-N-2-HFCC/CMC-NPs免疫后能够诱导产生更高滴度的血清抗体,能够显著提高淋巴细胞增殖,并且诱导产生了更高浓度的IL-2和IL-γ。这些研究结果表明:N-2-HFCC/CMC纳米颗粒是粘膜免疫的一种有效、安全的传递系统,且在医疗领域具有很大的应用潜力。
外文摘要:We have synthesized N-2-hydroxypropyl dimethylethyl ammonium chloride chitosan (N-2-HFCC) with higher water solubility than chitosan. To evaluate its potential as delivery carrier, NDV encapsulated in N-2-HFCC/CMC nanoparticles (NDV/La Sota-N-2-HFCC/CMC-NPs) with a diameter of 252.2 +/- 32.68 nm, Zeta potential of 41.1 +/- 0.89 mV, encapsulation efficiency of 96.6 +/- 0.72% and loading capacity of 53.2 +/- 1.11% were prepared. NDV/La Sota-N-2-HFCC/CMC-NPs are a novel type of attenuated live vaccine encapsulated in nanoparticles. These nanoparticles displayed lower cytotoxicity and higher stability. Their bioactivity was maintained when they were stored for three months at the room temperature. Release assay in vitro showed that NDV could be sustainably released after an initial burst release. In vivo immunization showed that immunization of specific pathogen free chickens with NDV/La Sota-N-2-HFCC/CMC-NPs intranasally induced high titers of serum antibody, significantly promoted lymphocyte proliferation and caused higher levels of interleukine-2 and interference-gamma. These results indicated that N-2-HFCC/CMC nanoparticles could serve as an efficient and safe delivery vehicle for mucosal immunity, and have a great potential in medical applications.
外文关键词:Newcastle disease; Live Newcastle disease vaccine; N-2-Hydroxypropyl dimethylethyl; ammonium chloride chitosan nanoparticles; Delivery carrier; Mucosal immune
作者: Jin, Z; Li, D; Dai, CX; Cheng, GG; Wang, XH; Zhao, K
作者单位:黑龙江大学
期刊名称:CARBOHYDRATE POLYMERS
期刊影响因子:4.811
出版年份:2017
出版刊次:9
点击下载:N-2-羟丙基二甲基乙基氯化铵壳聚糖纳米颗粒包裹的活新城疫疫苗的免疫反应分析
  1. 编译服务:动物支原体学
  2. 编译者:程金花
  3. 编译时间:2017-07-19