中文摘要：本研究分析了三种不同粘膜佐剂烷基糖苷（APG）、结兰胶和几丁聚糖（CS）与鼻内A/H1N1或B裂解流感疫苗联合免疫BALB/c小鼠的作用。结果表明，这三种佐剂都能扩大IgG和IgA免疫反应，与不使用佐剂的对照组相比提高小鼠的存活率。鸡肉注射能够刺激IgG的产生，但对IgA的浓度没有效果。APG是最有效的鼻内免疫佐剂，使用APG组的小鼠全部存活，且体重损失小、病毒滴度低、肺部病变程度低。这些结果表明，APG能够同时诱导系统免疫和粘膜免疫，是鼻内裂解流感A/H1N1 和B疫苗的有效佐剂。
外文摘要：Influenza viral infections are significant global public health concerns due to the morbidity and mortality associated with acute respiratory disease, secondary complications, and pandemic threats; thus, continuous efforts have been made to develop potent influenza vaccines. In this study, 3 different mucosal adjuvantsalkyl polyglycoside (APG), gellan gum, and chitosan (CS)were evaluated for their efficacy in intranasal A/H1N1 or B split influenza vaccines administered to BALB/c mice. Protective immunity was monitored by serum analysis for IgG, hemagglutination inhibition (HI), and neutralizing antibody levels, as well as mucosal IgA levels in nasal and pulmonary lavage fluids. Survival, body weight, lung viral titer, and pulmonary immunopathology were also examined following lethal influenza challenge. Notably, all adjuvants amplified the IgG and IgA immune responses (not detected in immunization of influenza B) and increased survival rate compared with controls administered adjuvant-free intranasal vaccines. Alternatively, intramuscular immunization stimulated IgG production, but had no effect on IgA levels. Our collective analysis identified that APG was the most effective intranasal adjuvant, as all mice survived influenza challenge with limited body weight loss, viral titer, and pulmonary pathology, similar to those observed with intramuscular vaccination. This evidence supports that APG can elicit both systemic and mucosal immunity, and may be an effective adjuvant in intranasal split influenza A/H1N1 and B vaccines.
外文关键词：alkyl polyglycoside； chitosan； gellan gum； intranasal immunization； split influenza vaccine
作者：Wu, H; Bao, YY; Wang, X; Zhou, DM; Wu, WZ
作者单位：药物制剂国家工程研究中心(上海)
期刊名称：HUMAN VACCINES & IMMUNOTHERAPEUTICS
期刊影响因子：2.157
出版年份：2017
出版刊次：6
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