中文摘要：本研究旨在分析0.20 mu m 颗粒物过滤器在降低摩托车尾气对气液临界培养的肺部上皮细胞毒性方面的作用。研究分别比较了在使用0.20 mu m 颗粒物过滤器和不使用0.20 mu m 颗粒物过滤器情况下的PM、一氧化碳、二氧化碳、总烃类、总挥发性有机物和氧化氮浓度。将肺部上皮细胞在ALI培养室中分别暴露于清洁空气、不带过滤器的摩托车尾气和带过滤器的摩托车尾气中，计算细胞相对活力和活性氧浓度。研究结果表明，ALI系统中增加0.20 mu m 颗粒物过滤器能够显著降低PM和THC的浓度，并通过提高暴露肺部上皮细胞的存活力和降低活性氧的生成发挥保护作用。
 
外文摘要：This study was designed to investigate whether the use of a 0.20 mu m particulate matter (PM) filter reduced the cytotoxicity induced by motorcycle exhaust (ME), a mixture of gases and particles, in lung epithelial cells cultured in air-liquid interface (ALI) inserts. The concentrations of PM, carbon monoxide, carbon dioxide, total hydrocarbons (THC), total volatile organic compounds, and nitrogen oxides in both filtered ME (fME) by a 0.20 mu m filter and non-filtered ME (non-fME) were measured. Lung epithelial cells were exposed to clean air, fME, or non-fME in the ALI chamber. Cell relative viabilities (CRV) and the reactive oxygen species (ROS) generation were determined. Our results revealed that PM2.5 was the main compound of PM in ME. After filtration, PM and THC levels were significantly reduced, as compared with non-fME. When compared with the clean air exposed group, the CRV in both fME and non-fME-exposed group was significantly reduced (p < 0.001), while their ROS generation were markedly increased (p < 0.001). When compared with non-fME-exposed group, the CRV and ROS generation were significantly improved following fME exposure (p < 0.05). As a result, of PM and THC concentrations were decreased approximately 90% and 22.71%, respectively, the CRV was improved from 40.4% (non-fME) to 55.7% (fME), and the increased ROS generation by non-fME was decreased about 51.6%. When BEAS-2B cells were exposed to fME, a time-dependent reduction in CRV was observed. In conclusion, our findings suggest that ME-exposure in the ALI system induces cytotoxicity and oxidative stress responses. The addition of a 0.20 mu m PM filter significantly modifies the particulate composition in PM and the concentration of THC, and shows protective effects by improving the survival of exposed lung epithelial cells and reducing the ROS generation. Therefore, emission factors such as different size of PM and THC from motorcycles may play a role in ME-induced toxicity.
外文关键词：Motorcycle exhausts; Air-liquid interface; Cytotoxicity; Particulate matter filter; Lung epithelial cells
作者：Yu, T; Zhang, XY; Zhong, L; Cui, Q; Hu, XY; Li, B; Wang, ZX; Dai, YF; Zheng, YX; Bin, P
作者单位：中国疾病预防控制中心
期刊名称：ENVIRONMENTAL POLLUTION
期刊影响因子：5.099
出版年份：2017
出版刊次：8
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