中文摘要：气液界面培养系统中，采用细胞性暴露程序是促进呼吸道细胞暴露于正常空气物质的一种有效方法，在该程序中细胞附着于多孔膜进行培养。这种暴露程序的稳定性和可重复性取决于细胞测试系统与暴露技术界面的优化设计。研究比较了CULTEX (R)线性和放射性两种测试气体进气模式的效果。在先前的研究过程中，主要采用线性设计的模块，即CULTEX (R)玻璃模块，能够满足ALI暴露研究的基本要求。不同气体分布的不稳定性促使研究者开发出一种新的暴露模块，其特征是细胞能够暴露于稳定的、可重复性的气溶胶中。基于此，研究者开发出了CULTEX RFS（细胞气液界面暴露系统）。本研究分析了两种不同暴露系统的颗粒分布和沉积特性，分析了放射性和线性气溶胶分布的优点和缺点。
 
外文摘要：The exposure of cellular based systems cultivated on microporous membranes at the air-liquid interface (ALI) has been accepted as an appropriate approach to simulate the exposure of cells of the respiratory tract to native airborne substances. The efficiency of such an exposure procedure with regard to stability and reproducibility depends on the optimal design at the interface between the cellular test system and the exposure technique. The actual exposure systems favor the dynamic guidance of the airborne substances to the surface of the cells in specially designed exposure devices. Two module types, based on a linear or radial feed of the test atmosphere to the test system, were used for these studies. In our technical history, the development started with the linear designed version, the CULTEX (R) glass modules, fulfilling basic requirements for running ALI exposure studies (Mohr and Durst, 2005). The instability in the distribution of different atmospheres to the cells caused us to create a new exposure module, characterized by a stable and reproducible radial guidance of the aerosol to the cells. The outcome was the CULTEX RFS (Mohr et al., 2010). In this study, we describe the differences between the two systems with regard to particle distribution and deposition clarifying the advantages and disadvantages of a radial to a linear aerosol distribution concept.
外文关键词：CULTEX (R) RFS; Air-liquid-interface; Inhalation exposure systems; Deposition efficiency
作者：Aufderheide, M; Heller, WD; Krischenowski, O; Mohle, N; Hochrainer, D
作者单位：德国卡尔泰克斯
期刊名称：EXPERIMENTAL AND TOXICOLOGIC PATHOLOGY
期刊影响因子：1.975
出版年份：2017
出版刊次：7
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