中文摘要：本研究的目的是分析IL-15作为肺炎链球菌抗原85A疫苗佐剂的作用。试验分别采用表达Ag85A-IL-15融合蛋白的质粒、空载体pcDNA3.1肌肉注射免疫C57BL/6小鼠，并以pcDNA3.1-Ag85A作为对照组。结果表明：采用pcDNA3.1-Ag85A-IL-15免疫的小鼠能够促进肺部产生更多的分泌性IgA(209 +/- 21 mu g/ml)，并激发更多的抗Ag85A的血浆IgG反应；免疫后能够上调IgG2a/IgG1比例，提升自杀性细胞的活性，促进小鼠Ag85A特异性脾T细胞增殖。采用pcDNA3.1-Ag85A-IL-15免疫能够促进脾组织中Th1型CD4(+) T细胞的极化，并显著上调血浆中一种典型的Th1细胞因子IFN-γ（458 +/- 98 pg/ml）的水平；同时，脾中IFN-γ表达的CD8(+)细胞浓度也增加了。
 
外文摘要：Objectives To investigate the potential of interleukin (IL)-15 as a novel adjuvant for Mycobacterium tuberculosis (Mtb) antigen 85A (Ag85A) vaccine. Results C57BL/6 mice were intramuscularly immunized three times with a plasmid expressing the Ag85A-IL-15 fusion protein (pcDNA3.1-Ag85A-IL15), with the empty pcDNA3.1 vector and the pcDNA3.1-Ag85A as control. Mice vaccinated with pcDNA3.1-Ag85A-IL-15 generated more secretory IgA (sIgA) into their lung (209 +/- 21 mu g/ml) and acquired an enhanced serum IgG response to Ag85A. IgG2a/IgG1 ratios were upregulated, natural killer cell activity was augmented and Ag85A-specific splenic T cell proliferation was enhanced in these mice as well. Vaccination with pcDNA3.1-Ag85A-IL-15 promoted the polarization of CD4(+) T cells towards a Th1 type in the spleen, and significantly upregulated the serum level of interferon (IFN)-gamma (458 +/- 98 pg/ml), a typical Th1 cytokine. IFN-gamma-expressing CD8(+) cells were also increased in the spleen after pcDNA3.1-Ag85A-IL-15 immunization. Conclusions A superior immune type I response in mice vaccinated with plasmid Ag85A-IL-15 has been achieved.
外文关键词：Antigen Ag85A； Interferon-gamma； IgG； Interleukin-15； Mycobacterium tuberulosis； Novel adjuvant； Th1 cells
作者：Sun, L; Yuan, Q; Xu, TH; Yao, L; Feng, JM; Ma, JF; Wang, LN; Lv, CL; Wang, DN Author Full Names: Sun, Li; Yuan, Quan; Xu, Tianhua; Yao, Li; Feng, Jiangmin; Ma, Jianfei; Wang, Lining; Lv, Changlong; Wang, Danan
作者单位：中国医科大学
期刊名称：BIOTECHNOLOGY LETTERS
期刊影响因子：1.73
出版年份：2017
出版刊次：8
点击下载：抗肺结核的新型佐剂：一种可激发小鼠强烈免疫反应、表达结核分枝杆菌抗原85A和IL-15融合蛋白的DNA疫苗