中文摘要：本研究分别从降低副作用和提高免疫保护性等方面评估了两种佐剂的作用。试验以虹鳟鱼为研究对象，免疫经福尔马林灭活、与CpG脱氧寡核苷酸组配的气单胞菌杀鲑亚种疫苗，将分别采用阳离子脂质体（CAF01）或弗氏不完全佐剂。结果表明，基于核苷酸和脂质体两种佐剂的疫苗不能消除副作用，但能够降低微观或宏观的不良影响；而且这两种疫苗能够达到与标准佐剂相同的保护作用。因此，这两种佐剂可以用于鱼类疫苗的免疫。
外文摘要：In an effort to reduce the frequency and severity of adverse reactions seen from the use of mineral oil adjuvants in salmonid fish, the effects of two alternative adjuvants were assessed, focusing on the induction of adverse effects as well as protection. Using rainbow trout (Oncorhynchus mykiss) as recipients, injection vaccines based on formalin-inactivated Aeromonas salmonicida subspecies salmonicida were formulated with CpG oligodeoxynucleotides, the liposomal cationic adjuvant formulation 01 (CAF01) or with Freund's incomplete adjuvant and administered intraperitoneally. Control groups of unvaccinated, Tris-buffered saline-injected or bacterin-injected individuals were included, and each group included in the study held a total number of 240 individuals. Subsequently, individuals from each group were examined for differences in Fulton's condition factor, macro-and microscopic pathological changes, as well as protection against experimental infection with A. salmonicida. While adverse effects were not eliminated, reductions in microscopic and macroscopic adverse effects, in particular, were seen for both the nucleotide-and liposome-based vaccine formulations. Furthermore, the induced protection appears similar to that of the benchmark formulation, thus introducing viable, potential alternative types of adjuvants for use in future fish vaccines.
外文关键词：SALMO-SALAR L; LEUKOCYTE CELL-LINE; ATLANTIC SALMON; INTRAPERITONEAL VACCINATION; SYSTEMIC AUTOIMMUNITY; ONCORHYNCHUS-MYKISS; BACTERIAL-DNA; CORD FACTOR; FURUNCULOSIS; RESPONSES
作者：Villumsen, KR; Koppang, EO; Christensen, D; Bojesen, AM
作者单位：丹麦哥本哈根大学
期刊名称：Villumsen, KR; Koppang, EO; Christensen, D; Bojesen, AM
期刊影响因子：3.9
出版年份：2017
出版刊次：7
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