中文摘要：研究重点分析了源于M1极化促炎性巨噬细胞的外胞体作为癌症疫苗增强剂的作用。结果表明，源于M1极化巨噬细胞的外胞体能够增强脂质磷酸钙（LCP）纳米颗粒包裹的Trp2疫苗的活性，能够诱导产生更强烈的抗体特异性细胞毒性T细胞反应。与CpG脱氧核糖核酸相比，M1外胞体更适合作为癌症疫苗的免疫增强剂。该研究表明，源于M1极化巨噬细胞的外胞体可用于制备疫苗佐剂。
 
外文摘要：Exosomes are small membrane-bound vesicular particles generated by most cells for intercellular communication and regulation. During biogenesis, specific lipids, RNAs, proteins, and carbohydrates are enriched and packaged into the vesicles so that the exosomal contents reflect not only the source but also the physiological conditions of the parental cells. These exosomes transport materials or signals to the target cells for diverse physiological purposes. Our study focused on the exosomes derived from Ml-polarized, proinflammatory macrophages for the possibility of using Ml exosomes as an immunopotentiator for a cancer vaccine. The MI exosomes displayed a tropism toward lymph nodes after subcutaneous injection, primarily taken up by the local macrophages and dendritic cells, and they induced the release of a pool of Thl cytokines. We found that Ml, but not M2, exosomes enhanced activity of lipid calcium phosphate (LCP) nanoparticle-encapsulated Trp2 vaccine, and they induced a stronger antigen-specific cytotoxic T cell response. The Ml exosomes proved to be a more potent immunopotentiator than CpG oligonucleotide when used with LCP nanoparticle vaccine in a melanoma growth inhibition study. Thus, our study indicated that exosomes derived from Ml-polarized macrophages could be used as a vaccine adjuvant.
外文关键词：CELL-DERIVED EXOSOMES; DENDRITIC CELLS; MICROVESICLES; VESICLES; DIFFERENTIATION; MELANOMA; ADJUVANT; RELEASE; POLARIZATION; METASTASIS
作者：Cheng, LF; Wang, YH; Huang, L
作者单位：北卡罗来纳大学
期刊名称：MOLECULAR THERAPY
期刊影响因子：6.688
出版年份：2017
出版刊次：7
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