中文摘要：本研究基于泰国两个农业省份2009年1月至2012年7月期间流感样疫苗监测数据随机，抽取了3000个患者的鼻咽拭子标本，采用RT-PCR技术对其中175个样本进行了肺炎支原体检测，多位点可变数目串联重复序列分析法（MLVA）对主要粘附蛋白（P1）进行了基因分型。结果表明，在所检测的样本中，97个样本为P1基因型1、60个为P1基因型2。在149个样本中检测到6个不同的多位点可变数目串联重复序列，其中优势序列为4/5/7/2 (40%) 和3/5/6/2 (26%)。对已知的大环内酯类耐药基因23S rRNA序列进行多态性分析表明，141个样本具有与大环内酯类抗生素的敏感性相关的基因型。
 
外文摘要：Studies on Mycoplasma pneumoniae in Thailand have focused on urban centers and have not included molecular characterization. In an attempt to provide a more comprehensive understanding of this organism, we conducted a systematic random sampling to identify 3,000 nasopharyngeal swab specimens collected from January 2009 through July 2012 during population-based surveillance for influenzalike illness in two rural provinces. M. pneumoniae was detected by real-time PCR in 175 (5.8%) specimens. Genotyping was performed using the major adhesion protein (P1) and multilocus variable-number tandem-repeat analysis (MLVA). Of the 157 specimens typed, 97 were P1 type 1 and 60 were P1 type 2. Six different MLVA profiles were identified in 149 specimens, with 4/5/7/2 (40%) and 3/5/6/2 (26%) predominating. There was no discrete seasonality to M. pneumoniae infections. Examination of the 23S rRNA sequence for known polymorphisms conferring macrolide resistance revealed that all 141 tested to possess the genotype associated with macrolide susceptibility.
外文关键词：Mycoplasma pneumoniae； community-acquired pneumonia； macrolide resistance； molecular epidemiology
作者：Whistler, T; Sawatwong, P; Diaz, MH; Benitez, AJ; Wolff, BJ; Sapchookul, P; Thamthitiwat, S; Winchell, JM
作者单位：泰国公共卫生部疾病控制中心
期刊名称：JOURNAL OF CLINICAL MICROBIOLOGY
期刊影响因子：3.712
出版年份：2017
出版刊次：7
点击下载：2009-2012年泰国两个不同乡村人群中肺炎支原体肺炎感染的分子鉴定