中文摘要：研究鉴定了一种氟苯基环修饰的aGalCer 类似物7DW8-5，该物质对CD1d分子表现出更高的亲和力，与aGalCer相比更具佐剂活性。在本研究中，将7DW8-5与表达约氏疟原虫环子孢子蛋白（PyCSP）的重组腺病毒联合进行肌肉注射，AdPyCS系统能够实现引流淋巴结（dLNs）中7DW8-5和PyCSP的共定位，尤其是在树状突细胞中。这种共现诱导了一系列串联事件，比如吸引DCs向引流淋巴结（dLNs）富集，并促进其激活和成熟，增强AdPyCS 诱导的DCs向CD8+ T cells分化的能力，最终促进产生佐剂作用，产生抗疟疾的保护作用。
 
外文摘要：A CD1d-binding, invariant (i) natural killer T (NICE)-cell stimulatory glycolipid, alpha-Galactosylceramide (alpha GalCer), has been shown to act as an adjuvant. We previously identified a fluorinated phenyl ring modified aGalCer analog, 7DW8-5, displaying a higher binding affinity for CD1d molecule and more potent adjuvant activity than aGalCer. In the present study, 7DW8-5 co-administered intramuscularly (i.m.) with a recombinant adenovirus expressing a Plasmodium yoelii circumsporozoite protein (PyCSP), AdPyCS, has led to a co-localization of 7DW8-5 and a PyCSP in draining lymph nodes (dLNs), particularly in dendritic cells (DCs). This occurrence initiates a cascade of events, such as the recruitment of DCs to dLNs and their activation and maturation, and the enhancement of the ability of DCs to prime CD8+ T cells induced by AdPyCS and ultimately leading to a potent adjuvant effect and protection against malaria.
外文关键词：Glycolipid adjuvant； CD1d； Dendritic cells； Lymph node； Adenovirus； Malaria vaccine； Co-localization； Protection； CD8+T cells； Intramuscular injection
作者：Li, XM; Huang, J; Kawamura, A; Funakoshi, R; Porcelli, SA; Tsuji, M
作者单位：洛克菲勒大学
期刊名称：VACCINE
期刊影响因子：3.235
出版年份：2017
出版刊次：5
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