中文摘要：本研究的目的是分析减毒鼠伤寒杆菌营养突变株（JOL912）作为活细菌疫苗载体的效率。结果表明，在pMMP65载体内，JOL912能稳定表达HA1基因，且能有效地将HA1蛋白传递到RAW264.7细胞内。采用JOL912载体免疫的鸡只能够显著提高HA1特异性IgG、sIgA抗体、IFN-γ、IL-6细胞因子的浓度和增强细胞免疫反应。体外试验表明，采用JOL912载体免疫的鸡只能够更快地从泄殖腔排出PR8病毒。该研究表明，基因工程JOL1635能够给鸡只建立起抗PR8病毒的特异性免疫原性和保护作用。
 
外文摘要：To evaluate the efficacy of attenuated Salmonella Typhimurium (JOL912) as a live bacterial vaccine vector. Materials & methods: The JOL912 engineered to deliver HA1 protein from influenza A/Puerto Rico/8/1934 (H1N1; PR8) virus was coined as JOL1635 and further evaluated for immunogenicity and protective efficacy. Results: The JOL1635 stably harbored the HA1 gene within pMMP65 plasmid with periplasmic expression and effective delivery of HA1 protein to RAW264.7 cells. The JOL1635 immunized chickens showed the significant increase in HA1-specific IgG, sIgA antibody, IFN-gamma, IL-6 cytokine and cellular immune responses. The postoral challenge, the JOL1635-immunized chickens showed a faster clearance of PR8 virus cloacal shedding than the control group. Conclusion: Generated JOL1635 can establish specific immunogenicity and protection against the PR8 virus in chickens.
外文关键词：chicken； influenza； LBV； Salmonella Typhimurium； vaccine
作者：Kamble, NM; Hyoung, KJ; Lee, JH
作者单位：韩国全北大学
期刊名称： FUTURE MICROBIOLOGY
期刊影响因子：3.374
出版年份：2017
出版刊次：7
点击下载：一种表达HA1蛋白免疫原性的减毒鼠伤寒杆菌营养突变株能够增强抗H1N1流感病毒的免疫原性和保护效率