中文摘要：本研究在优化条件下构建了一个包含百日咳博德特氏菌减毒株全细胞的海藻酸钙微球，分析了其免疫原性，并以小鼠为试验对象评估了这种疫苗微球的效率。研究构建的微球颗粒大小为151.1 ae m、多分散指数0.43、包封率89.6%、负荷容量36.3%，相对恒定释放率为15天。体外试验结果表明，与传统的海藻酸钙微球吸附疫苗相比，包含百日咳博德特氏菌的海藻酸钙微球吸附疫苗的效力更高（接近2.5倍）。该研究结果表明，百日咳博德特氏菌减毒株全细胞微囊化是提高wP疫苗质量的一种有效途径，尤其是在细菌浓度较低的情况下仍能获得较好的免疫原性。
 
外文摘要：There is no doubt about the whole cell pertussis vaccine efficacy, but it is necessary to improve the vaccine quality specially to decrease its toxicity by obtaining good immunogenicity with low bacterial content. In this work, under optimum condition inactivated B. pertussis bacteria cells entrapped with alginate microparticles were fabricated and in vivo immunogenicity and ptency of new microparticle based vaccine were evaluated in mice. Microspheres loaded with inactive B. pertussis bacterium cells were prepared via an emulsification method and analyzed for morphology, size, polydispersity index, loading efficiency, loading capacity, release profile and in vivo potency. The inactivated bacterial suspension mixture prepared in this work was nontoxic and showed potent ED50 (1: 333 of human dose) and preserved agglutinins 1, 2, 3. The optimum conditions for the preparation of microparticles were achieved at alginate concentration 3.8% (w/v), CaCl2 8% (w/v), PLL 0.1% (w/v), lipophilic surfactant 0.22 (% w/v), hydrophilic surfactant 3.6 (% w/v), cross linking time 3min, homogenization rate 600 rpm, and alginate to CaCl2 solution ratio 4. Both empty and B. pertussis loaded microparticles exhibited smooth surface texture and relatively spherical shape. The B. pertussis encapsulated microspheres fabricated under optimized conditions showed mean particle size 151.1 ae m, polydispersity index 0.43, loading efficiency 89.6%, loading capacity 36.3%, and relatively constant release rate lasted to 15 days. In vivo immunogenicity and protection study against wild type challenge showed strongly higher potency (approximately 2.5 fold) of encapsulated B. pertussis organisms than non-encapsulated conventional aluminum hydroxide adsorbed vaccine. It can be concluded that microencapsulation of inactive B. pertussis cells appears to be a suitable approach for improving the wP vaccine quality, specially by obtaining good immunogenicity with low bacterial content.
外文关键词：Bordetella pertussis； bacteria； microparticle； whole cell vaccine； potency； immunogenicity
作者：Dounighi, NM; Shahcheraghi, F; Razzaghi-Abyaneh, M; Nofeli, M; Zolfagharian, H
作者单位：伊朗巴斯德研究所
期刊名称：CURRENT PHARMACEUTICAL DESIGN
期刊影响因子：2.611
出版年份：2017
出版刊次：6
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