中文摘要：本研究旨在基于适宜鼻腔抗原传递的材料开发一种稳定的阳离子脂质体冻干粉。研究选用了包含DDA和TPGS的脂质体，测定了其大小和ZETA电位，开展了激光共聚焦显微镜细胞渗透性研究和细胞活性研究，采用卵清蛋白进行了初步的免疫研究和稳定性分析。制得的冻干粉大小合适，能够渗透鼻黏膜。体外试验表明，DDA和TPGS增加了脂质体的穿透性，胞内吸收好且活性稳定。与肌肉注射或对照组相比，制得的脂质体能够提高局部和阴道的免疫反应。该结果表明，所制备的新型脂质体可作为鼻腔疫苗传递载体进行推广应用，而且这种脂质体在液态状态下具有更好的热稳定性。
 
外文摘要：There is a pressing need for effective needle-free vaccines that are stable enough for use in the developing world and stockpiling. The inclusion of the cationic lipid DDA and the PEG-containing moiety TPGS into liposomes has the potential to improve mucosal delivery. The aim of this study was to develop stable lyophilized cationic liposomes based on these materials suitable for nasal antigen delivery. Liposomes containing DDA and TPGS were developed. Size and zeta potential measurements, ex vivo, CLSM cell penetration study and cell viability investigations were made. Preliminary immunisation and stability studies using ovalbumin were performed. The liposomes exhibited suitable size and charge for permeation across nasal mucosa. DDA and TPGS increased tissue permeation in ex vivo studies and cell uptake with good cell viability. The liposomes improved immune response both locally and vaginally when compared to i.m administration or control liposomes delivered nasally. Additionally, the lyophilized products demonstrated good stability in terms of Tg, size and antigen retention. This study has shown that the novel liposomes have potential for development as a mucosal vaccine delivery system. Furthermore, the stability of the lyophilized liposomes offers potential additional benefits in terms of thermal stability over liquid formats.
外文关键词：Liposomes； TPGS； DDA； Mucosal vaccine； Temperature stability； Nasal
作者：Yusuf, Helmy; Ali, Ahlam A.; Orr, Natalie; Tunney, Michael M.; McCarthy, Helen O.; Kett, Vicky L.
作者单位：贝尔法斯特皇后大学
期刊名称：INTERNATIONAL JOURNAL OF PHARMACEUTICS
期刊影响因子：4.24
出版年份：2017
出版刊次：11
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