中文摘要：研究基于细菌系统制备了重组HIV-1 Nef、Gp96和HMGB1 DNA，分析了应用两种外源佐剂pcDNA-HMGB1和pcDNA-Gp96 后包含重组HIV-1 Nef（pcDNA-Nef）DNA疫苗的免疫原性。结果表明，pcDNA-Nef与HMGB1 DNA联合免疫后能够诱导产生更高水平的IgG2a和IFN-γ介导的Th1免疫反应及细胞毒性T淋巴细胞活性。研究结果表明，全长HMGB1基因作为佐剂更能提高HIV DNA疫苗的治疗作用。
 
外文摘要：BACKGROUND: DNA immunization can induce long-term immune responses, which are required to design an effective HIV vaccine. It was shown that antigen-expressing plasmids can increase the protective immunity against infectious diseases such as: influenza and malaria. However, DNA-based immunizations have poor immunogenicity, thus the use of potent immunoadjuvants can enhance their potency. METHODS: In the current study, preparation of the recombinant HIV-1 Nef, Gp96 and HMGB1 DNA constructs was performed in bacterial system. Then, the immunogenicity of DNA construct harboring HIV-1 Nef gene (pcDNA-Nef) was studied using two endogenous adjuvants (pcDNA-HMGB1 and pcDNA-Gp96) in BALB/c mouse model. RESULTS: Our data showed that co-injection of pcDNA-Nef with pcDNA-HMGB1 effectively raised both humoral and cell-mediated immune responses in mice as compared to pcDNA-Nef adjuvanted with pcDNA-gp96. Indeed, co-immunization of HIV-1 Nef DNA with HMGB1 DNA significantly induced high levels of IgG2a and IFN-gamma directed toward Th1 responses and also cytotoxic T lymphocytes (CTLs) activity in comparison with other immunized groups. CONCLUSION: These findings suggest that the full length of HMGB1 gene could be a more efficient adjuvant for improvement of therapeutic HIV DNA-based immunization compared to the full length of gp96 gene.
 
外文关键词：HIV-1 Nef； Gp96； HMGB1； adjuvant； therapeutic vaccine
作者：Jafarzade, B. S.; Sadat, S. M.; Yaghobi, R.; Bolhassani, A.
作者单位：伊朗巴斯德研究所
期刊名称：BRATISLAVA MEDICAL JOURNAL-BRATISLAVSKE LEKARSKE LISTY
期刊影响因子：0.44
出版年份：2017
出版刊次：9
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