中文摘要：本研究的目的是评估口服表面携带流感HA1/L/ AcmA重组蛋白、LL-HA1/L/AcmA蛋白的非重组乳酸乳球菌在诱导小鼠粘膜免疫反应和形成抗流感病毒保护方面的作用。结果表明，口服LL-HA1/L/AcmA的小鼠排泄物、小肠洗液、支气管肺泡灌洗液和鼻腔洗液中能够检测到特异性sIgA。结果表明，小鼠口服LL-HA1/L/AcmA能够诱导呼吸道和胃肠道产生粘膜反应。LL-HA1/L/AcmA保护效率的试验表明，未接受免疫的小鼠对流感病毒表现出高度的易感性，而免疫组7/8的小鼠生存了下来。结论：口服LL-HA1/L/AcmA能够诱导小鼠产生粘膜反应，更重要的是能够产生抗致死性流感病毒的保护作用。研究着重分析了乳酸乳球菌在流感疫苗传递方面的潜在应用价值。
外文摘要：Mucosal immunization of influenza vaccine is potentially an effective approach for the prevention and control of influenza. The objective of the present study was to evaluate the ability of oral immunization with a non-recombinant Lactococcus lactis displaying HA1/L/AcmA recombinant protein, LL-HA1/L/AcmA, to induce mucosal immune responses and to accord protection against influenza virus infection in mice. The LL-HA1/LIAcmA was orally administered into mice and the immune response was evaluated. Mice immunized with LL-HA1/L/AcmA developed detectable specific sIgA in faecal extract, small intestine wash, BAL fluid and nasal fluid. The results obtained demonstrated that oral immunization of mice with LL-HA1/L/AcmA elicited mucosal immunity in both the gastrointestinal tract and the respiratory tract. The protective efficacy of LL-HA1/L/AcmA in immunized mice against a lethal dose challenge with influenza virus was also assessed. Upon challenge, the non-immunized group of mice showed high susceptibility to influenza virus infection. In contrast, 7/8 of mice orally immunized with LL-HA1/L/AcmA survived. In conclusion, oral administration of LL-HA1/L/AcmA in mice induced mucosal immunity and most importantly, provided protection against lethal influenza virus challenge. These results highlight the potential application of L. lactis as a platform for delivery of influenza virus vaccine.
外文关键词：CROSS-PROTECTION; RESPIRATORY-TRACT; IGA ANTIBODIES; ESCHERICHIA-COLI; SECRETORY IGA; A VIRUSES; SUBUNIT-B; VACCINES; VACCINATION; INFECTION
作者：Jee, PF; Tiong, V; Shu, MH; Khoo, JJ; Wong, WF; Rahim, RA; AbuBakar, S; Chang, LY Author Full Names: Jee, Pui-Fong; Tiong, Vunjia; Shu, Meng-Hool; Khoo, Jing-Jing; Wong, Won Fen; Rahim, Raha Abdul; AbuBakar, Sazaly; Chang, Li-Yen
作者单位：马来亚大学
期刊名称：PLOS ONE
期刊影响因子：3.11
出版年份：2017
出版刊次：11
点击下载：口服表面携带流感HA1抗原的非重组乳酸乳球菌能够诱导小鼠的粘膜免疫