中文摘要：本研究制备了一种壳聚糖衍生物N-磷酸壳聚糖，该化合物水溶性好且在卵清蛋白蛋白抗原模型中呈现出较好的肌肉注射疫苗传递作用。基于N-磷酸壳聚糖的抗原组合物可以促进抗原从注射部位向第二淋巴器官脾扩散。进一步的免疫试验表明，1 mg/mlN-磷酸壳聚糖疫苗组合能够显著提高抗原特异性免疫反应，包括在免疫小鼠脾细胞中产生更高的抗原特异性IgG抗体滴度、淋巴细胞转化率和细胞因子（γ-干扰素、白细胞介素10和4）分泌水平。从研究结果可知，水溶性壳聚糖衍生物N-磷酸壳聚糖作为一种抗原载体在免疫预防和治疗领域具有潜在的应用前景。
 
外文摘要：Chitosan, as a potential vaccine delivery material, has obtained much attention for immunization prevention and therapy. However, its poor water solubility brings inconvenience for the practical applications. To address this issue, researchers have carried out many chemical modifications to prepare water-soluble chitosan derivatives for vaccine delivery. In this work, we prepared a chitosan derivative N-phosphonium chitosan with excellent water solubility and explored its potential as an intramuscular vaccine delivery system by using ovalbumin as a model antigen. Different vaccine formulations were intramuscularly injected into test mice. Through an immunohistochemistry assay, N-phosphonium chitosan-based antigen formulation could promote antigen arrival from injection site to the secondary lymph organ spleen. Further immunization results showed that 1 mg/ml N-phosphonium chitosan-based vaccine formulation could contribute to significantly higher level of antigen-specific immune responses, including higher antigen-specific IgG antibody titer, splenocyte proliferation, and cytokines secretion (interferon-gamma, interleukin-10, and interleukin-4) by the splenocytes of the immunized mice. From the results, the water-soluble chitosan derivative N-phosphonium chitosan could be developed as a potential antigen carrier for immunization prevention and therapy.
外文关键词：N-phosphonium chitosan；intramuscular immunization；vaccine delivery system
作者：Cai, JZ; Zhang, W; Xu, JK; Xue, W; Liu, ZH
作者单位：广东暨南大学
期刊名称：JOURNAL OF BIOMATERIALS APPLICATIONS
期刊影响因子：2.24
出版年份：2017
出版刊次：11
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