中文摘要：研究采用颗粒凝集（PA）抗体反应和环介导等温扩增（LAMP）基因检测技术分析了免疫色谱法快速诊断肺炎支原体的敏感性和特异性。研究分析了165个样本的感染情况，其中有59个样本的患者血清颗粒凝集（PA）抗体反应升高了4倍。在首次检测的拭子中，LAMP法检测到了60个阳性样本，免疫色谱检测到49个阳性样本；与PA抗体反应、LAMP法相比，免疫色谱法的敏感性和特异性分别为81.4% (48/59) 、99.1% (105/106)，81.7% (49/60) 、100% (105/105)；同一样本，咽拭子的敏感性显著高于鼻拭子。在首次检测中，免疫色谱法测定咽拭子的结果较好。
 
外文摘要：The sensitivity and specificity of a new rapid Mycoplasma pneumoniae antigen immunochromatography (IC) test, DK-MP-001, were determined using particle agglutination (PA) antibody response and loop-mediated isothermal amplification (LAMP) gene detection as the gold standard. Of 165 patients, 59 were diagnosed with M. pneumoniae infection based on a fourfold rise of serum PA antibody during the course of the illness. Of the first visit swabs, 60 were positive for M. pneumoniae on LAMP, and 49 were positive for M. pneumoniae antigen on IC test. Compared with PA antibody and LAMP, the sensitivity/specificity of the IC test were 81.4% (48/59) and 99.1% (105/106); and 81.7% (49/60) and 100% (105/105), respectively. IC test detected antigen in pharyngeal swabs more sensitively than in nasal swabs for the same subjects (P<0.05). The IC test performs well enough to be used with pharyngeal swabs at the first examination.
外文关键词：immunochromatography； Mycoplasma pneumoniae； Mycoplasma pneumoniae pneumonia； rapid diagnosis
作者：Onari, S; Okada, T; Okada, T; Okano, S; Kakuta, O; Kutsuma, H; Kobayashi, M; Kondo, Y; Sakaguchi, N; Tajima, T; Nagao, M; Nakayama, E; Niimi, R; Nishimura, S; Higashidate, Y; Hikita, T; Meguro, H; Mori, T; Yoto, Y; Tsutsumi, H
作者单位：日本札幌医科大学
期刊名称：PEDIATRICS INTERNATIONAL
期刊影响因子：0.88
出版年份：2017
出版刊次：10
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