中文摘要：本研究根据变量投影重要性（VIP）采用变量选择法鉴定肺炎支原体品系的拉曼谱带的重要性。同时，对缺失P40/P90蛋白或P1和P40/P90蛋白的突变体进行变量投影重要性分析，这些突变体与特定的拉曼谱带重要性相关，能够区分具有特定支原体表面蛋白组成的不同的基因型。结果表明，变量选择及其与特定支原体表面组成的相关性，是进行肺炎支原体检测与分型的重要步骤。
 
外文摘要：Mycoplasma pneumoniae is a human respiratory tract pathogen causing chronic bronchitis and atypical or walking pneumonia. The major surface protein P1 must form complexes with proteins P30 and P40/P90 to function in receptor binding and gliding motility, and variability in P1 and P40/P90 distinguishes the two major M. pneumoniae genotypes. Strains belonging to each genotype can be differentiated with high sensitivity and specificity by utilizing surface-enhanced Raman spectroscopy on silver nanorod arrays. Here, we used the variable selection method of variable importance in projection (VIP) to identify Raman bands important in M. pneumoniae strain classification. Furthermore, VIP analysis of mutants lacking P40/P90, or P1 and P40/P90, correlated certain Raman bands important in distinguishing genotypes, with specific mycoplasma surface protein composition and presentation. Variable selection, and its correlation with specific mycoplasma surface components, is an important next step in developing this platform for M. pneumoniae detection and genotyping.
外文关键词：Genotyping； Mycoplasma pneumoniae； nanorod array； surface-enhanced Raman spectroscopy； variable importance in projection
作者：Krause, DC; Hennigan, SL; Henderson, KC; Clark, HJ; Dluhy, RA
作者单位：佐治亚大学
期刊名称：ANALYTICAL LETTERS
期刊影响因子：1.17
出版年份：2017
出版刊次：15
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