中文摘要：研究将肽聚糖结合域（PA）与霍乱毒素A1亚单元（CTA1）融合，将该融合蛋白导入枯草芽胞杆菌，并在培养基中培养。一步法纯化CTA1并通过GEM颗粒进行展示，从而形成CTA1-GEM复合物，以该复合物作为流感疫苗佐剂，分析其对小鼠免疫反应的影响。结果表明，CTA1-GEM复合物能够增强特异性机体（IgG）和粘膜（IgA）免疫反应，诱导细胞免疫反应，是一种可行的粘膜佐剂。
 
外文摘要：The A1 subunit of cholera toxin (CTA1) retains the adjuvant function of CT, without its toxic side effects, making the molecule a promising mucosal adjuvant. However, the methods required to obtain a pure product are both complicated and expensive, constricting its potential commercial applicability. Here, we fused the peptidoglycan binding domain (PA) to the C-terminus of CTA1, which enabled the fusion protein to be expressed by Bacillus subtilis, and secreted into the culture medium. CTA1 was then purified and displayed on GEM particles using a one step process, which resulted in the formation of CTA1-GEM complexes. Next, the CTA1-GEM complexes were used as an adjuvant to enhance the immune responses of mice to the influenza subunit vaccine. It was observed that the CTA1-GEM complexes enhanced specific systemic (IgG) and mucosal (IgA) immune responses against antigen, and induced cellular immune responses as well. The data presented here suggests that CTA1-GEM complexes can serve as a viable mucosal adjuvant.
外文关键词：Cholera toxin A1 subunit； Surface display； Adjuvant； GEM particles； Lactococcus lactis
作者：Zhang, YP; Yu, XM; Hou, LT; Chen, J; Li, PC; Qiao, XW; Zheng, QS; Hou, JB
作者单位：江苏省农业科学院
期刊名称：PROTEIN EXPRESSION AND PURIFICATION
期刊影响因子：1.46
出版年份：2018
出版刊次：1
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