中文摘要：结核分枝杆菌表面成分在调控宿主免疫反应方面具有重要作用。本研究分析了合成了脂甘露聚糖（LM）主链糖，这种糖是结核分枝杆菌表面的核心葡聚糖。研究基于聚合法建立了吡喃甘露聚糖快速合成化学方法，其核心结构即是脂甘露聚糖（LM）。可以通过特定单体浓度、多功能三环原酸酯甘露糖单体的立体结构和区域选择性控制LM葡聚糖的大小。研究分析了合成吡喃甘露聚糖的免疫特性及其作为佐剂的适用性。结果表明，吡喃甘露聚糖发挥佐剂功能的机理可能与NF-kappa B、炎性体通路相关。
 
外文摘要：Surface components of Mycobacterium tuberculosis (Mtb) play crucial roles in modulating host immune responses. Thorough understandings of immunological properties of the Mtb's surface components are essential for the development of tuberculosis treatment and prevention. Unfortunately, the accessibility to the molecules on the surface of Mtb is limited by the structural complexity due to their various macromolecular nature and the hazard of culturing Mtb. In this study, we reveal a practical synthesis of lipomannan (LM) backbone polysaccharides - the core glycans found on Mtb's surface. A rapid synthetic approach based on a controlled polymerization was developed for the chemical synthesis of mannopyranans, the core structure of LM. The size of the LM glycans can be controlled by using specific monomer concentrations in addition to stereo-and regioselectivity derived from the versatile tricyclic orthoester mannose monomer. The immunological properties of the synthesized mannopyranans were investigated and their adjuvant potential was revealed. The adjuvanticity mechanism of the synthetic mannopyranans appears to involve the NF-kappa B and inflammasome pathways.
外文关键词： mannan back-bone polysaccharides； mannosyl tricyclic orthoester monomer； Mycobacterium tuberculosis surface； lipomannan； vaccine adjuvant
作者：Wattanasiri, C; Paha, J; Ponpuak, M; Ruchirawat, S; Boonyarattanakalin, S
作者单位：泰国法政大学
期刊名称：CARBOHYDRATE POLYMERS
期刊影响因子：5.15
出版年份：2017
出版刊次：11
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