中文摘要：本研究的目的是分析支气管肺泡灌洗液（BALF）中的细胞因子并探索出可用于预测儿童支原体肺炎（MPP）严重性的指示因子。研究分析了150个肺炎支原体或气管异物（FB）儿童样本。结果表明，肺炎支原体肺炎严重的患者IL17A的mRNA表达水平显著低于轻微肺炎支原体肺炎患者或气管异物患者。IL17A表达水平与胸腔积液、顽固性等临床指标相关；顽固性肺炎支原体或者出现胸腔积液的患者儿童体内IL17A表达水平较低。此外，严重肺炎支原体肺炎儿童胸腔积液中IL17A的蛋白水平大幅度降低。因此，胸腔积液中IL17A水平的降低能够作为一种生物标记较好地预测儿童肺炎支原体肺炎严重程度。
 
外文摘要：Early distinction between severe Mycoplasma pneumoniae pneumonia (MPP) and mild MPP is still difficult. The aim of this study was to analyze cytokines in bronchoalveolar lavage fluid (BALF) and explore predicting factors of severe MPP in children. Retrospective analysis was performed on 150 children with MPP or bronchial foreign body (FB) admitted in our hospital. The mRNA levels of IL17A were found significantly lower in severe MPP group comparing with mild MPP group or FB group. However, no significant difference was found in the levels of IL4, IL10 or interferon beta1 (IFN beta 1) between the two groups. Receiver operator characteristic (ROC) curve analysis showed that IL17A can be used to distinguish severe MPP from mild MPP. These results were confirmed in a validation cohort including 40 MPP children from another hospital. IL17A levels were correlated with some clinical characters, such as refractoriness and pleural effusion. Lower IL17A levels were more likely to be found in refractory MPP children or in MPP children with pleural effusion. Moreover, the protein levels of IL17A in BALF were also found greatly decreased in children with severe MPP. Thus, decreased IL17A levels in BALF may be a valuable biomarker to identify severe MPP in children.
外文关键词：INNATE LYMPHOID-CELLS; METHYLPREDNISOLONE PULSE THERAPY; BRONCHOALVEOLAR LAVAGE FLUID; COMMUNITY-ACQUIRED PNEUMONIA; IL-17-PRODUCING T-CELLS; GENE-EXPRESSION; NEUTROPHIL RECRUITMENT; CLINICAL-FEATURES; INTERFERON-GAMMA; IL-17 PRODUCTION
作者：Yang, Mingyue; Meng, Fanzheng; Wang, Kuo; Gao, Man; Lu, Ruihua; Li, Mengyao; Zhao, Fangxing; Huang, Lijuan; Zhang, Yining; Cheng, Genhong; Wang, Xiaosong
作者单位：吉林大学医院
期刊名称：SCIENTIFIC REPORTS
期刊影响因子：4.63
出版年份：2017
出版刊次：11
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