中文摘要：已有报道表明，支原体感染与癌症迁移、入侵、上皮细胞-间充质细胞转换以及对核苷类似物化疗药物抗性相关。本研究表明，清除支原体感染会增强肝癌细胞对氯氨铂、吉西他滨和米托蒽醌的敏感性。采用ANXA2多肽的N端阻断宿主细胞中猪鼻支原体膜蛋白P37与膜联蛋白A2之间的互作能够增强HCC97L细胞对吉西他滨和米托蒽醌的敏感性。同时，去除支原体后，未观察到转运蛋白及三磷酸腺苷结合盒蛋白B1、C1和G2与多药物抗性相关的分布或表达的变化。这些结果表明，支原体诱导肝癌细胞产生对多种药物的抗性，这一过程需要P37与膜联蛋白A2互作作用的参与，这种互作下游路径还需要通过进一步的研究进行探讨。
 
外文摘要： Mycoplasma infection has been reported to be associated with cancer migration, invasion, epithelial-mesenchymal transition as well as the resistance to nucleoside analogues chemotherapeutic drugs. In this study, we found that the sensitivity of hepatocarcinoma cells to Cisplatin, Gemcitabine and Mitoxantrone was increased by mycoplasma elimination. Similar to the effect of anti-mycoplasma agent, interrupting the interaction between Mycoplasma hyorhinis membrane protein P37 and Annexin A2 of host cells using the N-terminal of ANXA2 polypeptide enhanced the sensitivity of HCC97L cells to Gemcitabine and Mitoxantrone. Meanwhile, we did not observe any changes in expression or distribution of multidrug resistance associated transporters, ATP-Binding Cassette protein B1, C1 and G2, on the removal of mycoplasma. These results suggest that mycoplasma induces a resistance to multiple drugs in hepatocarcinoma cells which required the interaction of P37 and Annexin A2. The pathway downstream this interaction needs to be explored.
外文关键词：MOUSE SARCOMA-CELLS; GASTRIC-CANCER; MEMBRANE GLYCOPROTEIN; HYORHINIS INFECTION; LIPOPROTEIN P37; GUT MICROBIOTA; IN-VITRO; INVASIVENESS; TRANSPORTERS; PROTEINS
作者：Liu, DY; Hu, Y; Guo, Y; Zhu, Z; Lu, BZ; Wang, XL; Huang, YJ
作者单位：中山大学
期刊名称：PLOS ONE
期刊影响因子：3.11
出版年份：2017
出版刊次：10
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