中文摘要：研究分析了藏猪肺炎支原体肺炎流行病学，并采用酶联免疫吸附剂测定法（ELISA）、PCR和16s rRNA测序技术对肺炎支原体进行了检测。在被屠宰的155头猪种，有29头出现了炎性病理症状，通过肺部石蜡切片法和苏木精-伊红染色确诊了病灶淋巴细胞被入侵。采用PCR技术测定了屠宰藏猪的支气管肺泡灌洗液（BALF）和其它群体的鼻拭子。结果表明，2014年10-12月期间，四川、西藏和青海3个地区藏猪群体中流行的肺炎支原体的比例为20.48%（93/454），不同年龄阶段的流行特点存在显著差异。繁殖母猪（45.83%，22/48）和仔猪（50%，3/6）群体中能够检测到抗肺炎支原体抗体。对BALF的PCR扩增和凝胶结果表明，6.45% (10/155)的猪只呈现肺炎支原体阳性。繁殖母猪和仔猪群体血浆中肺炎支原体的浓度高于其它群体。
 
外文摘要：Enzootic pneumonia (EP), often caused by Mycoplasma hyopneumoniae, occurs in Tibetan pigs between October and December in Western China. The aim of this study was to determine the prevalence of M. hyopneumoniae in Tibetan pig herds and also the prevalence of infection. M. hyopneumoniae was detected using enzyme-linked immunosorbent assay, polymerase chain reaction (PCR), and 16S rRNA sequencing. Twenty-nine inflammatory gross-lesions were observed in 155 lungs of slaughtered pigs. Invasion of focal lymphocytes was confirmed by paraffin sectioning and hematoxylin-eosin staining of lung sections. Bronchoalveolar lavage fluid (BALF) from slaughtered Tibetan pigs and nasal swabs from others were assayed using PCR. The prevalence of M. hyopneumoniae in Tibetan pig herds (via ELISA) was 20.48% (93/454) in 3 provinces (Sichuan, Tibet autonomous region, and Qinghai) between October and December of 2014. The difference in prevalence among animals in six different growing stages was statistically significant (P < 0.05). Anti-M. hyopneumoniae antibody was detected in breeding sows (45.83%; 22/48) and piglets (50%; 3/6). PCR and gel electrophoresis of BALF showed that 6.45% (10/155) of pigs were positive for M. hyopneumoniae. The presence of M. hyopneumoniae in serum was higher in piglets and breeding sows than in any other group. In conclusion, the results of this study showed that M. hyopneumoniae is prevalent among Tibetan pigs between October and December in Western China. To the authors' knowledge, this is the first investigation of M. hyopneumoniae prevalence in Tibetan pigs of Western China using serological tests, PCR, and 16S rRNA sequencing.
外文关键词：Tibetan pig； Mycoplasma hyopneumoniae； Seroprevalence； Elisa； PCR； BALF
作者：Qiu, G; Rui, YP; Li, K; Huang, SC; Han, ZQ; Wang, XQ; Jiang, WT; Luo, HQ; Lan, YF; Li, JK
作者单位：西藏农牧学院
期刊名称：TROPICAL ANIMAL HEALTH AND PRODUCTION
期刊影响因子：1.1
出版年份：2017
出版刊次：10
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