中文摘要：研究合成了一种抗野生H5N1病毒的新型禽流感疫苗，该疫苗采用腺病毒表达的粒细胞-单核细胞集落刺激因子(GM-CSF)作为佐剂。结果表明，腺病毒表达的重组GM-CSF (rGM-CSF)，也称为rAd-GM-CSF能够提高抗AIV的血细胞凝集抑制（HI）滴度；加入rGM-CSF能够显著增加肺部IFN-、IL-4和主要组织相容性复合物II的表达水平。因此，采用rGM-CSF作为灭活AIV疫苗的佐剂能够产生高水平的HI滴度、高浓度的免疫相关的分子表达水平并能提供更高的保护效率。
 
外文摘要：Wild birds play a key role in the spread of avian influenza virus (AIV). There is a continual urgent requirement for AIV vaccines to address the ongoing genetic changes of AIV. In the current study, we trialed a novel AIV vaccine against the wild bird source of H5N1 type AIV with recombinant adenovirus expressing granulocyte monocyte colony-stimulating factor (GM-CSF) as an adjuvant. A total of 150-day-old commercial chicks, with AIV-maternal-derived antibody, were divided into 6 groups. The primary vaccination was performed at day 14 followed by a subsequent boosting and intramuscular challenge on day 28 and 42, respectively. Recombinant GM-CSF (rGM-CSF) expressed by adenovirus, named as rAd-GM-CSF, raised the hemagglutination inhibition (HI) titers (log(2)) against AIV from 7.0 (vaccinate with inactivated vaccine alone) to 8.4 after booster immunization. Moreover, the rGM-CSF addition markedly increased the expression of interferon-, interleukin-4, and major histocompatibility complex-II in the lungs, compared with those immunized with inactivated vaccine alone on day 29, that is, 18h post booster immunization. Following challenge, chicks inoculated with the inactivated AIV vaccine and rAd-GM-CSF together exhibited mild clinical signs and 62% survivals compared to 33% in the group immunized with inactivated AIV vaccine alone. Higher level of HI titers, immune related molecule expressions, and protection ratio demonstrates a good potential of rGM-CSF in improving humoral and cell mediated immune responses of inactivated AIV vaccines.
外文关键词：wild bird； H5N1； vaccine； adjuvant； GM-CSF
作者：Wang, XW; Wang, XL; Jia, YQ; Wang, CY; Tang, QX; Han, QS; Xiao, S; Yang, ZQ
作者单位：西北农林科技大学
期刊名称：JOURNAL OF INTERFERON AND CYTOKINE RESEARCH
期刊影响因子：2.26
出版年份：2017
出版刊次：10
点击下载：重组腺病毒表达的粒细胞-单核细胞集落刺激因子与灭活H5N1禽流感疫苗共同免疫能够增强对野生H5N1病毒的免疫反应和保护效率