中文摘要：抗原特异性免疫反应决定了单纯疱疹病毒2型（HSV-2）疫苗的效率。为优化抗原gD2的免疫原性，研究开发了一种融合编码HSV-2糖蛋白D和编码病毒衣壳蛋白UL25基因的gD2UL25 DNA疫苗，并以福尔马林-灭活HSV-2 (FI-HSV-2)疫苗作为对照。结果表明，与gD2相比，gD2UL25能够诱导产生更高水平的免疫保护作用。应用gD2UL25进一步评价IL28B佐剂的作用。结果表明，与单独使用gD2UL2相比，采用gD2UL25、IL28B联合疫苗能够诱导产生更强的体液和T细胞免疫。与对照组相比，gD2UL25/IL28B降低了HSV-2病毒负载浓度，并诱导产生了抗生殖道病变的保护作用。这些结果表明，将gD2UL25 DNA疫苗与IL28B佐剂联合进行预防接种能够增强体液免疫和T细胞免疫反应，提高雌性小鼠抗HSV-2的免疫保护反应。
 
外文摘要：Antigen-specific immune responses determine the efficacy of herpes simplex virus type 2 (HSV-2) vaccines. To optimize the immunogenicity of the antigen gD2, we developed the gD2UL25 DNA vaccine encoding HSV-2 glycoprotein D and UL25 gene encoding viral capsid vertex proteins in this study. The gD2 and gD2UL25 DNA vaccines were compared with formalin-inactivated HSV-2 (FI-HSV-2), and results showed a greater protective immune response induced by gD2UL25 than by gD2. Therefore, gD2UL25 was chosen to evaluate further using the IL28B adjuvant. Immunization with gD2UL25/IL28B elicited stronger humoral and T cell immune responses than with gD2UL25 alone. Compared with controls, gD2UL25/IL28B decreased HSV-2 viral loads and induced protective effects against genital tract lesions generated by HSV-2. These findings demonstrated that the prophylactic DNA vaccine gD2UL25 with IL28B adjuvant could enhance the humoral and T cell immune responses, and improve the protective immune response against HSV-2 in female mice compared with FI-HSV-2.
外文关键词：herpes simplex virus type 2； DNA vaccine； adjuvant IL28B； glycoprotein D； UL25； protective immune response
作者：Zhou, Y; Wang, ZY; Xu, YQ; Zhang, ZQ; Hua, R; Liu, W; Jiang, CL; Chen, Y; Yang, WY; Kong, W
作者单位：吉林大学
期刊名称：VIRAL IMMUNOLOGY
期刊影响因子：1.26
出版年份：2017
出版刊次：10
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